Academic Journal
Radiolabeling, Quality Control and In Vivo Imaging of Multimodal Targeted Nanomedicines
| العنوان: | Radiolabeling, Quality Control and In Vivo Imaging of Multimodal Targeted Nanomedicines |
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| المؤلفون: | Nguyen, Phuoc-Vinh, Allard-Vannier, Emilie, Aubrey, Nicolas, Labrugère-Sarroste, Christine, Chourpa, Igor, Sobilo, Julien, Le Pape, Alain, Hervé-Aubert, Katel |
| المساهمون: | Nanomédicaments et Nanosondes (NMNS), Université de Tours (UT), Vietnam National University, Ho Chi Minh City / Đại học Quốc gia TP. Hồ Chí Minh (VNU-HCM), Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Aquitaine de Caractérisation des Matériaux (PLACAMAT), Université de Bordeaux (UB)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre d'Imagerie du Petit Animal (CIPA), Université d'Orléans (UO), Transgenèse et archivage d'animaux modèles (TAAM), Centre National de la Recherche Scientifique (CNRS), Cancéropôle Grand Ouest (Emergence CGO 2019 NANOTIF project), ANR-10-LABX-0053,MAbImprove,Optimization of therapeutic monoclonal antibodies development Better antibodies, better developed AND better used(2010) |
| المصدر: | ISSN: 1999-4923 ; Pharmaceutics ; https://hal.inrae.fr/hal-03925149 ; Pharmaceutics, 2022, 14 (12), pp.2679. ⟨10.3390/pharmaceutics14122679⟩ ; https://www.mdpi.com/1999-4923/14/12/2679. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2022 |
| المجموعة: | Université François-Rabelais de Tours: HAL |
| مصطلحات موضوعية: | EGFR, SPECT-CT, in vivo tracking, nanomedicine, radiolabeling, scFv, [SDV.IB]Life Sciences [q-bio]/Bioengineering, [SDV.CAN]Life Sciences [q-bio]/Cancer |
| الوصف: | International audience ; Following our previous study on the development of EGFR-targeted nanomedicine (NM-scFv) for the active delivery of siRNA in EGFR-positive cancers, this study focuses on the development and the quality control of a radiolabeling method to track it in in vivo conditions with nuclear imaging. Our NM-scFv is based on the electrostatic complexation of targeted nanovector (NV-scFv), siRNA and two cationic polymers. NV-scFv comprises an inorganic core, a fluorescent dye, a polymer layer and anti-EGFR ligands. To track NM-scFv in vivo with nuclear imaging, the DTPA chemistry was used to radiolabel NM-scFv with 111In. DTPA was thiolated and introduced onto NV-scFv via the maleimide chemistry. To obtain suitable radiolabeling efficiency, different DTPA/NV-scFv ratios were tested, including 0.03, 0.3 and 0.6. At the optimized ratio (where the DTPA/NV-scFv ratio was 0.3), a high radiolabeling yield was achieved (98%) and neither DTPA-derivatization nor indium-radiolabeling showed any impact on NM-scFv’s physicochemical characteristics (DH ~100 nm, PDi < 0.24). The selected NM-scFv-DTPA demonstrated good siRNA protection capacity and comparable in vitro transfection efficiency into EGFR-overexpressing cells in comparison to that of non-derivatized NM-scFv (around 67%). Eventually, it was able to track both qualitatively and quantitatively NM-scFv in in vivo environments with nuclear imaging. Both the radiolabeling and the NM-scFv showed a high in vivo stability level. Altogether, a radiolabeling method using DTPA chemistry was developed with success in this study to track our NM-scFv in in vivo conditions without any impact on its active targeting and physicochemical properties, highlighting the potential of our NM-scFv for future theranostic applications in EGFR-overexpressing cancers. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/36559172; hal-03925149; https://hal.inrae.fr/hal-03925149; https://hal.inrae.fr/hal-03925149/document; https://hal.inrae.fr/hal-03925149/file/2022_Nguyen_pharmaceutics_vol14_art-02679.pdf; PUBMED: 36559172; WOS: 000903393300001 |
| DOI: | 10.3390/pharmaceutics14122679 |
| الاتاحة: | https://hal.inrae.fr/hal-03925149 https://hal.inrae.fr/hal-03925149/document https://hal.inrae.fr/hal-03925149/file/2022_Nguyen_pharmaceutics_vol14_art-02679.pdf https://doi.org/10.3390/pharmaceutics14122679 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.DC5A58F4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/pharmaceutics14122679 |
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