Academic Journal
Efficient transfection of Xenobiotic Responsive Element-biosensor plasmid using diether lipid and phosphatidylcholine liposomes in differentiated HepaRG cells
| العنوان: | Efficient transfection of Xenobiotic Responsive Element-biosensor plasmid using diether lipid and phosphatidylcholine liposomes in differentiated HepaRG cells |
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| المؤلفون: | Demazeau, Maxime, Quesnot, Nicolas, Ripoche, Nicolas, Rauch, Claudine, Jeftić, Jelena, Morel, Fabrice, Gauffre, Fabienne, Benvegnu, Thierry, Loyer, Pascal |
| المساهمون: | Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Nutrition, Métabolismes et Cancer (NuMeCan), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was funded by the Agence Nationale de la Recherche (ANR, contract NISTEC ANR-09-CESA-003-002), the Institut National de la santé et de la Recherche Médicale (Inserm) and the Centre National de la Recherche Scientifique (CNRS)., ANR-09-CESA-0003,NISTEC,Nouvelles stratégies in vitro pour l'évaluation de la cytotoxicite et la genotoxicite des contaminants de l'environnement(2009) |
| المصدر: | ISSN: 0378-5173 ; International Journal of Pharmaceutics ; https://univ-rennes.hal.science/hal-01518045 ; International Journal of Pharmaceutics, 2017, 524 (1-2), pp.268-278. ⟨10.1016/j.ijpharm.2017.03.080⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2017 |
| المجموعة: | Université de Rennes 1: Publications scientifiques (HAL) |
| مصطلحات موضوعية: | archaeolipids, biosensor plasmid, diether lipids, HepaRG cells, Liposomes, Transfection, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology |
| الوصف: | International audience ; In this study, we evaluated cationic liposomes prepared from diether-NH2 and egg phosphatidylcholine (EPC) for in vitro gene delivery. The impact of the lipid composition, i.e. the EPC and Diether-NH2 molar ratio, on in vitro transfection efficiency and cytotoxicity was investigated using the human HEK293T and hepatoma HepaRG cells known to be permissive and poorly permissive cells for liposome-mediated gene transfer, respectively. Here, we report that EPC/Diether-NH2-based liposomes enabled a very efficient transfection with low cytotoxicity compared to commercial transfection reagents in both HEK293T and proliferating progenitor HepaRG cells. Taking advantage of these non-toxic EPC/Diether-NH2-based liposomes, we developed a method to efficiently transfect differentiated hepatocyte-like HepaRG cells and a biosensor plasmid containing a Xenobiotic Responsive Element and a minimal promoter driving the transcription of the luciferase reporter gene. We demonstrated that the luciferase activity was induced by a canonical inducer of cytochrome P450 genes, the benzo[a]pyrene, and two environmental contaminants, the fluoranthene, a polycyclic aromatic hydrocarbon, and the endosulfan, an organochlorine insecticide, known to induce toxicity and genotoxicity in differentiated HepaRG cells. In conclusion, we established a new efficient lipofection-mediated gene transfer in hepatocyte-like HepaRG cells opening new perspectives in drug evaluation relying on xenobiotic inducible biosensor plasmids. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/28365389; hal-01518045; https://univ-rennes.hal.science/hal-01518045; https://univ-rennes.hal.science/hal-01518045/document; https://univ-rennes.hal.science/hal-01518045/file/Demazeau%20et%20al.%20-%20Efficient%20Transfection%20of%20Xenobiotic%20Responsive%20El.pdf; PRODINRA: 419106; PUBMED: 28365389; WOS: 000401108500026 |
| DOI: | 10.1016/j.ijpharm.2017.03.080 |
| الاتاحة: | https://univ-rennes.hal.science/hal-01518045 https://univ-rennes.hal.science/hal-01518045/document https://univ-rennes.hal.science/hal-01518045/file/Demazeau%20et%20al.%20-%20Efficient%20Transfection%20of%20Xenobiotic%20Responsive%20El.pdf https://doi.org/10.1016/j.ijpharm.2017.03.080 |
| Rights: | http://creativecommons.org/licenses/by-sa/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.DBBE7151 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ijpharm.2017.03.080 |
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