Academic Journal
Probing the Assembly of HDL Mimetic, Drug Carrying Nanoparticles Using Intrinsic Fluorescence
| العنوان: | Probing the Assembly of HDL Mimetic, Drug Carrying Nanoparticles Using Intrinsic Fluorescence |
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| المؤلفون: | Raut, Sangram, Garud, Ashwini, Nagarajan, Bhavanai, Sabnis, Nirupama, Remaley, Alan, Fudala, Rafal, Gryczynski, Ignacy, Gryczynski, Zygmunt, Dzyuba, Sergei V., Borejdo, Julian, Lacko, Andras |
| المصدر: | The Journal of Pharmacology and Experimental Therapeutics |
| بيانات النشر: | ASPET |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Apolipoprotein-A-I, Protein Secondary Structure, Synthetic Peptide Analogs, High-Density-Lipoproteins, Cholesterol Efflux, Antiinflammatory Properties, Poor-Prognosis, Delivery, Atherosclerosis, Doxorubicin |
| الوصف: | Reconstituted high-density lipoprotein (HDL) containing apolipoprotein A-I (Apo A-I) mimics the structure and function of endogenous (human plasma) HDL due to its function and potential therapeutic utility in atherosclerosis, cancer, neurodegenerative diseases, and inflammatory diseases. Recently, a new class of HDL mimetics has emerged, involving peptides with amino acid sequences that simulate the the primary structure of the amphipathic alpha helices within the Apo A-I protein. The findings reported in this communication were obtained using a similar amphiphilic peptide (modified via conjugation of a myristic acid residue at the amino terminal aspartic acid) that self-assembles (by itself) into nanoparticles while retaining the key features of endogenous HDL. The studies presented here involve the macromolecular assembly of the myristic acid conjugated peptide (MYR-5A) into nanomicellar structures and its characterization via steady-state and time-resolved fluorescence spectroscopy. The structural differences between the free peptide (5A) and MYR-5A conjugate were also probed, using tryptophan fluorescence, Förster resonance energy transfer (FRET), dynamic light scattering, and gel exclusion chromatography. To our knowledge, this is the first report of a lipoprotein assembly generated from a single ingredient and without a separate lipid component. The therapeutic utility of these nanoparticles (due to their capablity to incorporate a wide range of drugs into their core region for targeted delivery) was also investigated by probing the role of the scavenger receptor type B1 in this process. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://doi.org/10.1124/jpet.119.262899; https://repository.tcu.edu/handle/116099117/43807; https://jpet.aspetjournals.org/content/373/1/113 |
| DOI: | 10.1124/jpet.119.262899 |
| الاتاحة: | https://repository.tcu.edu/handle/116099117/43807 https://doi.org/10.1124/jpet.119.262899 https://jpet.aspetjournals.org/content/373/1/113 |
| Rights: | Public Domain (US Government Work) ; Public Domain |
| رقم الانضمام: | edsbas.DBA85E40 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1124/jpet.119.262899 |
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