Academic Journal
Generation of reactive oxygen species is not involved in idarubicin‐induced apoptosis in human leukaemic cells
| العنوان: | Generation of reactive oxygen species is not involved in idarubicin‐induced apoptosis in human leukaemic cells |
|---|---|
| المؤلفون: | Liu, Feng‐Ting, Kelsey, Stephen M., Newland, Adrian C., Jia, Li |
| المصدر: | British Journal of Haematology ; volume 115, issue 4, page 817-825 ; ISSN 0007-1048 1365-2141 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2001 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | The anthracycline antibiotic idarubicin (IDA) induces double‐stranded DNA breaks, the generation of reactive oxygen species (ROS) and apoptosis in human leukaemic cells. It is unclear whether the generation of ROS is associated with the apoptotic process. Using the T‐lymphoblastic leukaemic CEM cell line, we found that IDA‐induced DNA breaks were correlated with final cell death. The reduction in mitochondrial membrane potential (ΔΨm) and the generation of ROS occurred simultaneously with IDA‐induced activation of caspase‐9 and caspase‐3. Inhibition of caspases by a pan‐caspase inhibitor, benzyloxycarbonyl‐Val‐Ala‐Asp‐fluoromethyl ketone (Z‐VAD‐fmk) completely blocked IDA‐induced reduction of ΔΨm, apoptosis and final cell death. Interestingly, ROS generation was significantly enhanced by Z‐VAD‐fmk. ROS generation was neither caspase dependent nor part of the apoptotic process. IDA‐mediated reduction in ΔΨm is caspase dependent and is not a consequence of the generation of ROS. These results indicate that IDA‐induced generation of ROS and apoptosis are separate events. Inhibition of caspases facilitates IDA‐mediated generation of ROS. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1046/j.1365-2141.2001.03216.x |
| الاتاحة: | http://dx.doi.org/10.1046/j.1365-2141.2001.03216.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2141.2001.03216.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2141.2001.03216.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.DB64AEB3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1046/j.1365-2141.2001.03216.x |
|---|