التفاصيل البيبلوغرافية
| العنوان: |
MAPK signaling is necessary for neurogenesis in Nematostella vectensis |
| المؤلفون: |
Layden, Michael, Johnston, Hereroa, Amiel, Aldine, Havrilak, Jamie, Steinworth, Bailey, Chock, Taylor, Röttinger, Eric, Martindale, Mark |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2016 |
| المجموعة: |
BioMed Central |
| الوصف: |
Background The nerve net of Nematostella is generated using a conserved cascade of neurogenic transcription factors. For example, NvashA , a homolog of the achaete-scute family of basic helix-loop-helix transcription factors, is necessary and sufficient to specify a subset of embryonic neurons. However, positive regulators required for the expression of neurogenic transcription factors remain poorly understood. Results We show that treatment with the MEK/MAPK inhibitor U0126 severely reduces the expression of known neurogenic genes, Nvath-like , NvsoxB(2) , and NvashA , and known markers of differentiated neurons, suggesting that MAPK signaling is necessary for neural development. Interestingly, ectopic NvashA fails to rescue the expression of neural markers in U0126-treated animals . Double fluorescence in situ hybridization and transgenic analysis confirmed that NvashA targets represent both unique and overlapping populations of neurons. Finally, we used a genome-wide microarray to identify additional patterning genes downstream of MAPK that might contribute to neurogenesis. We identified 18 likely neural transcription factors, and surprisingly identified ~40 signaling genes and transcription factors that are expressed in either the aboral domain or animal pole that gives rise to the endomesoderm at late blastula stages. Conclusions Together, our data suggest that MAPK is a key early regulator of neurogenesis, and that it is likely required at multiple steps. Initially, MAPK promotes neurogenesis by positively regulating expression of NvsoxB(2) , Nvath-like , and NvashA . However, we also found that MAPK is necessary for the activity of the neurogenic transcription factor NvashA . Our forward molecular approach provided insight about the mechanisms of embryonic neurogenesis. For instance, NvashA suppression of Nvath-like suggests that inhibition of progenitor identity is an active process in newly born neurons, and we show that downstream targets of NvashA reflect multiple neural subtypes rather than ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
http://www.biomedcentral.com/1741-7007/14/61 |
| الاتاحة: |
http://www.biomedcentral.com/1741-7007/14/61 |
| Rights: |
Copyright 2016 Layden et al. |
| رقم الانضمام: |
edsbas.DB54B846 |
| قاعدة البيانات: |
BASE |