Academic Journal
Crystal structure of the C67A mutant of isopentenyl diphosphate isomerase complexed with a mechanism-based irreversible inhibitor
| العنوان: | Crystal structure of the C67A mutant of isopentenyl diphosphate isomerase complexed with a mechanism-based irreversible inhibitor |
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| المؤلفون: | Wouters, Johan, Oudjama, Yamina, Stalon, Victor, Droogmans, Louis, Poulter, C. Dale |
| المصدر: | Proteins, 54 (2 |
| سنة النشر: | 2004 |
| المجموعة: | DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
| مصطلحات موضوعية: | Sciences bio-médicales et agricoles, Binding Sites, Carbon-Carbon Double Bond Isomerases -- antagonists & inhibitors, Carbon-Carbon Double Bond Isomerases -- chemistry, Carbon-Carbon Double Bond Isomerases -- genetics, Carbon-Carbon Double Bond Isomerases -- metabolism, Crystallography, X-Ray, Enzyme Inhibitors -- chemistry, Enzyme Inhibitors -- metabolism, Enzyme Inhibitors -- pharmacology, Epoxy Compounds -- chemistry, Epoxy Compounds -- metabolism, Epoxy Compounds -- pharmacology, Escherichia coli -- enzymology, Escherichia coli -- genetics, Isomerism, Organophosphorus Compounds -- chemistry, Organophosphorus Compounds -- metabolism, Organophosphorus Compounds -- pharmacology, Point Mutation -- genetics, Protons |
| الوصف: | Isopentenyl diphosphate:dimethylallyl diphosphate (IPP:DMAPP) isomerase is a key enzyme in the biosynthesis of isoprenoids. The mechanism of the isomerization reaction involves protonation of the unactivated carbon-carbon double bond in the substrate. Analysis of the 1.97 Å crystal structure of the inactive C67A mutant of E. coli isopentenyl diphosphate:dimethylallyl diphosphate isomerase complexed with the mechanism-based inactivator 3,4-epoxy-3-methyl-1-butyl diphosphate is in agreement with an isomerization mechanism involving Glu 116, Tyr 104, and Cys 67. In particular, the results are consistent with a mechanism where Glu116 is involved in the protonation step and Cys67 in the elimination step. © 2003 Wiley-Liss, Inc. ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. ; FLWIN ; info:eu-repo/semantics/published |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 2 full-text file(s): application/pdf | application/pdf |
| اللغة: | English |
| Relation: | uri/info:doi/10.1002/prot.10573; uri/info:pmid/14696183; uri/info:scp/0346458808; local/VX-005383; https://dipot.ulb.ac.be/dspace/bitstream/2013/50473/5/202d1568-00f8-40e1-b97f-3817728853bb.txt; https://dipot.ulb.ac.be/dspace/bitstream/2013/50473/3/50473.pdf; http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/50473 |
| الاتاحة: | http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/50473 https://dipot.ulb.ac.be/dspace/bitstream/2013/50473/5/202d1568-00f8-40e1-b97f-3817728853bb.txt https://dipot.ulb.ac.be/dspace/bitstream/2013/50473/3/50473.pdf |
| رقم الانضمام: | edsbas.DB031A07 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |