التفاصيل البيبلوغرافية
| العنوان: |
Data_Sheet_1_Construction and Analysis of a ceRNA Network in Cardiac Fibroblast During Fibrosis Based on in vivo and in vitro Data.PDF |
| المؤلفون: |
Qing-Yuan Gao (10013927), Hai-Feng Zhang (3059928), Zhi-Teng Chen (3072327), Yue-Wei Li (10014767), Shao-Hua Wang (1490677), Zhu-Zhi Wen (10014770), Yong Xie (283717), Jing-Ting Mai (10014773), Jing-Feng Wang (5118854), Yang-Xin Chen (5118863) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Genetics, Genetic Engineering, Biomarkers, Developmental Genetics (incl. Sex Determination), Epigenetics (incl. Genome Methylation and Epigenomics), Gene Expression (incl. Microarray and other genome-wide approaches), Genome Structure and Regulation, Genomics, Genetically Modified Animals, Livestock Cloning, Gene and Molecular Therapy, cardiac fibroblast, fibrosis, competing endogenous RNA, ADAM metallopeptidase domain 19, transforming growth factor beta induced |
| الوصف: |
Aims Activation of cardiac fibroblasts (CF) is crucial to cardiac fibrosis. We constructed a cardiac fibroblast-related competing endogenous RNA (ceRNA) network. Potential functions related to fibrosis of “hub genes” in this ceRNA network were explored. Materials and Methods The Gene Expression Omnibus database was searched for eligible datasets. Differentially expressed messenger (m)RNA (DE-mRNA) and long non-coding (lnc)RNA (DE-lncRNA) were identified. microRNA was predicted and validated. A predicted ceRNA network was constructed and visualized by Cytoscape, and ceRNA crosstalk was validated. A Single Gene Set Enrichment Analysis (SGSEA) was done, and the Comparative Toxicogenomics Database (CTD) was employed to analyze the most closely associated pathways and diseases of DE-mRNA in the ceRNA network. The functions of DE-mRNA and DE-lncRNA in the ceRNA network were validated by small interfering (si)RNA depletion. Results The GSE97358 and GSE116250 datasets (which described differentially expressed genes in human cardiac fibroblasts and failing ventricles, respectively) were used for analyses. Four-hundred-and-twenty DE-mRNA and 39 DE-lncRNA, and 369 DE-mRNA and 93 DE-lncRNA were identified, respectively, in the GSE97358 and GSE116250 datasets. Most of the genes were related to signal transduction, cytokine activity, and cell proliferation. Thirteen DE-mRNA with the same expression tendency were overlapped in the two datasets. Twenty-three candidate microRNAs were predicted and the expression of 11 were different. Only two DE-lncRNA were paired to any one of 11 microRNA. Finally, two mRNA [ADAM metallopeptidase domain 19, (ADAM19) and transforming growth factor beta induced, (TGFBI)], three microRNA (miR-9-5p, miR-124-3p, and miR-153-3p) and two lncRNA (LINC00511 and SNHG15) constituted our ceRNA network. siRNA against LINC00511 increased miR-124-3p and miR-9-5p expression, and decreased ADAM19 and TGFBI expression, whereas siRNA against SNHG15 increased miR-153-3p and decreased ADAM19 expression. ADAM19 and ... |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Data_Sheet_1_Construction_and_Analysis_of_a_ceRNA_Network_in_Cardiac_Fibroblast_During_Fibrosis_Based_on_in_vivo_and_in_vitro_Data_PDF/13621139 |
| DOI: |
10.3389/fgene.2020.503256.s001 |
| الاتاحة: |
https://doi.org/10.3389/fgene.2020.503256.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.DAEECE93 |
| قاعدة البيانات: |
BASE |