Academic Journal
Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis
| العنوان: | Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis |
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| المؤلفون: | Silveira, Tony L. R., Martins, Gabriel B., Domingues, William B., Remiao, Mariana H., Barreto, Bruna F., Lessa, Ingrid M., Santos, Lucas, Pinhal, Danillo, Dellagostin, Odir A., Seixas, Fabiana K., Collares, Tiago, Robaldo, Ricardo B., Campos, Vinicius F. |
| المساهمون: | Universidade Estadual Paulista (UNESP) |
| بيانات النشر: | Frontiers Media Sa |
| سنة النشر: | 2018 |
| المجموعة: | Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
| مصطلحات موضوعية: | acclimation, blood, brackish water, fish, freshwater, genes, salt, transfer |
| الوصف: | Ministerio da Ciencia ; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) ; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) ; Ministerio da Ciencia: 422292/2016-8 ; CNPq: 472210/2013-0 ; CAPES: AUXPE 2900/2014 ; Silversides are fish that inhabit marine coastal waters, coastal lagoons, and estuarine regions in southern South America. The freshwater (FW) silversides have the ability to tolerate salinity variations. Odontesthes humensis have similar habitats and biological characteristics of congeneric O. bonariensis, the most studied silverside species and with great economic importance. Studies revealed that O. bonariensis is not fully adapted to FW, despite inhabiting hyposmotic environments in nature. However, there is little information about stressful environments for cultivation of silverside O. humensis. Thus, the aim of this study was to evaluate the stress and osmoregulation responses triggered by the osmotic transfers on silverside O. humensis. Silversides were acclimated to FW (0 ppt) and to brackish water (BW, 10 ppt) and then they were exposed to opposite salinity treatment. Silverside gills and blood were sampled on pre-transfer (D0) and 1, 7, and 15 days (D1, D7, and D15) after changes in environmental salinity, the expression levels of genes atp1a3a, slc12a2b, kcnh1, and hspa1a were determined by quantitative reverse transcription-PCR for evaluation of osmoregulatory and stress responses. Furthermore, glycemia, hematocrit, and osmolality were also evaluated. The expression of atp1a3a was up-and down-regulated at D1 after the FW-BW and BW-FW transfers, respectively. Slc12a2b was up-regulated after FW-BW transfer. Similarly, kcnh1 and hspa1a were up-regulated at D1 after the BW-FW transfer. O. humensis blood osmolality decreased after the exposure to FW. It remained stable after exposure to BW, indicating an efficient hyposmoregulation. The glycemia had a peak at D1 after BW-FW transfer. No changes were observed in hematocrit. The return to the pre-transfer ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1664-8021 |
| Relation: | Frontiers In Genetics; 2,274; http://dx.doi.org/10.3389/fgene.2018.00028; Frontiers In Genetics. Lausanne: Frontiers Media Sa, v. 9, 10 p., 2018.; http://hdl.handle.net/11449/163813; WOS:000424244300002; WOS000424244300002.pdf |
| DOI: | 10.3389/fgene.2018.00028 |
| الاتاحة: | http://hdl.handle.net/11449/163813 https://doi.org/10.3389/fgene.2018.00028 |
| Rights: | openAccess |
| رقم الانضمام: | edsbas.DAE77B58 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16648021 |
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| DOI: | 10.3389/fgene.2018.00028 |