التفاصيل البيبلوغرافية
| العنوان: |
Comparison between different RNA extraction methods. |
| المؤلفون: |
Maria Jose Lista (10979241), Pedro M. Matos (11436764), Thomas J. A. Maguire (11436767), Kate Poulton (8943914), Elena Ortiz-Zapater (3708226), Robert Page (6598751), Helin Sertkaya (11436770), Ana M. Ortega-Prieto (11436773), Edward Scourfield (11436776), Aoife M. O’Byrne (11436779), Clement Bouton (11436782), Ruth E. Dickenson (11436785), Mattia Ficarelli (11436788), Jose M. Jimenez-Guardeño (5658871), Mark Howard (436694), Gilberto Betancor (6029015), Rui Pedro Galao (10979235), Suzanne Pickering (513611), Adrian W. Signell (9415767), Harry Wilson (11436791), Penelope Cliff (360440), Mark Tan Kia Ik (9415773), Amita Patel (848633), Eithne MacMahon (170039), Emma Cunningham (11220916), Katie Doores (3565958), Monica Agromayor (11436794), Juan Martin-Serrano (11052702), Esperanza Perucha (429856), Hannah E. Mischo (11436797), Manu Shankar-Hari (3576134), Rahul Batra (171339), Jonathan Edgeworth (471617), Mark Zuckerman (11436800), Michael H. Malim (9415812), Stuart Neil (5715038), Rocio Teresa Martinez-Nunez (11436803) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Genetics, Molecular Biology, Biotechnology, Immunology, Cancer, Hematology, Infectious Diseases, Virology, Biological Sciences not elsewhere classified, Information Systems not elsewhere classified, sops freely available, show significant differences, sensitive although overall, provide multiple workflows, also compared one, 90 ° c, 70 ° c, step master mix, step go lo, perform manual processing, low viral titres, bind viral dna, 2 testing portable, rna 96 kit, findings make sars, div >< p, detect viral n, beckman performing better |
| الوصف: |
(A) Dilution curve of the positive control provided by IDT (plasmid containing SARS-CoV-2 N gene) using N1 or N2 primer-probe sets with the Taqman FastVirus mix. A semi-log regression was used to calculate the coefficient of determination (R 2 ). (B, C) A set of four swab samples were used for RNA extraction with the indicated kit. RT-qPCR was run with N1 and N2 primer-probe sets employing the FastVirus (B) or the Luna Master mixes (C). Shapiro-Wilk test was used for normality assessment prior to analysis employing ANOVA (parametric data) or Friedman test (non-parametric data). These samples were previously classified as positive (CPS) by the diagnostics lab, the number indicates different donors. Dots represent each individual RT-qPCR technical duplicate, line connects average of replicates and statistics were performed in average duplicates. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Comparison_between_different_RNA_extraction_methods_/16623299 |
| DOI: |
10.1371/journal.pone.0256813.g002 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0256813.g002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.DA767F6C |
| قاعدة البيانات: |
BASE |