Academic Journal
Selective gene dosage by CRISPR‐Cas9 genome editing in hexaploid Camelina sativa
| العنوان: | Selective gene dosage by CRISPR‐Cas9 genome editing in hexaploid Camelina sativa |
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| المؤلفون: | Morineau, Céline, Bellec, Yannick, Tellier, Frédérique, Gissot, Lionel, Kelemen, Zsolt, Nogué, Fabien, Faure, Jean‐Denis |
| المساهمون: | Agence Nationale de la Recherche |
| المصدر: | Plant Biotechnology Journal ; volume 15, issue 6, page 729-739 ; ISSN 1467-7644 1467-7652 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2017 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | In many plant species, gene dosage is an important cause of phenotype variation. Engineering gene dosage, particularly in polyploid genomes, would provide an efficient tool for plant breeding. The hexaploid oilseed crop Camelina sativa, which has three closely related expressed subgenomes, is an ideal species for investigation of the possibility of creating a large collection of combinatorial mutants. Selective, targeted mutagenesis of the three delta‐12‐desaturase ( FAD 2 ) genes was achieved by CRISPR ‐Cas9 gene editing, leading to reduced levels of polyunsaturated fatty acids and increased accumulation of oleic acid in the oil. Analysis of mutations over four generations demonstrated the presence of a large variety of heritable mutations in the three isologous Cs FAD 2 genes. The different combinations of single, double and triple mutants in the T3 generation were isolated, and the complete loss‐of‐function mutants revealed the importance of delta‐12‐desaturation for Camelina development. Combinatorial association of different alleles for the three FAD 2 loci provided a large diversity of Camelina lines with various lipid profiles, ranging from 10% to 62% oleic acid accumulation in the oil. The different allelic combinations allowed an unbiased analysis of gene dosage and function in this hexaploid species, but also provided a unique source of genetic variability for plant breeding. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/pbi.12671 |
| الاتاحة: | http://dx.doi.org/10.1111/pbi.12671 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fpbi.12671 https://onlinelibrary.wiley.com/doi/pdf/10.1111/pbi.12671 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.DA2FCF3D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/pbi.12671 |
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