Academic Journal
Advancing Multiple Detection in RT-LAMP with a Specific Probe Assembled from Plural Three-Way-Junction Structures
| العنوان: | Advancing Multiple Detection in RT-LAMP with a Specific Probe Assembled from Plural Three-Way-Junction Structures |
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| المؤلفون: | Yi Sun, Sicai Zhang, Lijuan Qi, Xiaojun Zhang, Meiting Yang, Zhijun Guo, Zhenxin Wang, Yan Du |
| سنة النشر: | 1753 |
| مصطلحات موضوعية: | Biochemistry, Medicine, Microbiology, Cell Biology, Genetics, Molecular Biology, Immunology, Developmental Biology, Infectious Diseases, Space Science, Biological Sciences not elsewhere classified, Mathematical Sciences not elsewhere classified, reliable diagnostic methods, offering significant potential, minimum detectable concentration, mediated isothermal amplification, exhibits excellent stability, multiple probe units, salmonella typhimurium <, salmonella enteritidis <, advancing multiple detection, time fluorescent probe, specific probe assembled, hp enable single, accurate disease detection, robust multivariate probes, 3 , salmonella <, specific probes, hexagonal probe |
| الوصف: | The timely detection of diseases and the accurate identification of pathogens require the development of efficient and reliable diagnostic methods. In this study, we have developed a novel specific multivariate probe termed MRTFP (multivariate real-time fluorescent probe) by assembling strand exchange three-way-junction (3WJ) structures. The 3WJ structures were incorporated into a four-angle probe (FP) and a hexagonal probe (HP), to target the multivariate genes of Salmonella . The FP and HP enable single-step and multiplexed detection in RT-LAMP (real-time loop-mediated isothermal amplification) with exceptional sensitivity and specificity. Encouragingly, real food samples contaminated with Salmonella ( Salmonella enteritidis and Salmonella typhimurium ) can be readily identified and distinguished with a minimum detectable concentration (MDC) of 10 3 CFU/mL without the need for further culture. The introduction of MRTFP allows for simultaneous detection of dual or three targets in a single tube for LAMP, thereby improving detection efficiency. The MRTFP simplifies the design of robust multivariate probes, exhibits excellent stability, and avoids interference from multiple probe units, offering significant potential for the development of specific probes for efficient and accurate disease detection and pathogen identification. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Advancing_Multiple_Detection_in_RT-LAMP_with_a_Specific_Probe_Assembled_from_Plural_Three-Way-Junction_Structures/24579196 |
| DOI: | 10.1021/acs.analchem.3c03877.s001 |
| الاتاحة: | https://doi.org/10.1021/acs.analchem.3c03877.s001 https://figshare.com/articles/journal_contribution/Advancing_Multiple_Detection_in_RT-LAMP_with_a_Specific_Probe_Assembled_from_Plural_Three-Way-Junction_Structures/24579196 |
| Rights: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.DA0CF396 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.analchem.3c03877.s001 |
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