Academic Journal
Flow cytometric detection and microscopic observation of activated eosinophils in peripheral blood
| العنوان: | Flow cytometric detection and microscopic observation of activated eosinophils in peripheral blood |
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| المؤلفون: | Nishikawa, Satomi, Matsuhiroya, Shiori, Kono, Mari |
| المصدر: | Microscopy ; volume 72, issue 3, page 265-269 ; ISSN 2050-5698 2050-5701 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2022 |
| الوصف: | Eosinophils possess highly electron-dense granules with crystal-like structures and are characterized as high side scatter (SSC) areas by flow cytometry analysis. Eosinophils with low SSC features have been noted in extremely rare cases; however, the underlying cause remains unclear. Eosinophils in the low SSC area were analyzed using microscopy. A transmission electron microscope revealed the loss of crystal-like structures in granules with low electron density and piecemeal degranulation, which was undetectable by May–Grünwald–Giemsa staining. Based on the results of flow cytometry, May–Grünwald–Giemsa staining and transmission electron microscopy, SSC values could help potentially detect crystal-like structures and piecemeal degranulation eosinophils. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/jmicro/dfac060 |
| DOI: | 10.1093/jmicro/dfac060/47850549/dfac060.pdf |
| الاتاحة: | https://doi.org/10.1093/jmicro/dfac060 https://academic.oup.com/jmicro/advance-article-pdf/doi/10.1093/jmicro/dfac060/47850549/dfac060.pdf https://academic.oup.com/jmicro/article-pdf/72/3/265/50561954/dfac060.pdf |
| Rights: | https://academic.oup.com/pages/standard-publication-reuse-rights |
| رقم الانضمام: | edsbas.D9F29AC0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/jmicro/dfac060 |
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