Academic Journal
Increased basal insulin secretion in Pdzd2-deficient mice
| العنوان: | Increased basal insulin secretion in Pdzd2-deficient mice |
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| المؤلفون: | Tsang, SW, Shao, D, Cheah, KSE, Okuse, K, Yao, KM, Leung, PS |
| بيانات النشر: | //www.elsevier.com/locate/mce Ireland |
| سنة النشر: | 2010 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | KATP channel, Glucose-stimulated insulin secretion, Gene-trap mutagenesis, Pdzd2, INS-1E, Blood Glucose - metabolism, Nerve Tissue Proteins - genetics - metabolism, Mice, Knockout, Insulin-Secreting Cells - cytology - metabolism, Insulin - blood - secretion |
| الوصف: | Expression of the multi-PDZ protein Pdzd2 (PDZ domain-containing protein 2) is enriched in pancreatic islet β cells, but not in exocrine or α cells, suggesting a role for Pdzd2 in the regulation of pancreatic β-cell function. To explore the in vivo function of Pdzd2, Pdzd2-deficient mice were generated. Homozygous Pdzd2 mutant mice were viable and their gross morphology appeared normal. Interestingly, Pdzd2-deficient mice showed enhanced glucose tolerance in intraperitoneal glucose tolerance tests and their plasma insulin levels indicated increased basal insulin secretion after fasting. Moreover, insulin release from mutant pancreatic islets was found to be twofold higher than from normal islets. To verify the functional defect in vitro, Pdzd2 was depleted in INS-1E cells using two siRNA duplexes. Pdzd2-depleted INS-1E cells also displayed increased insulin secretion at low concentrations of glucose. Our results provide the first evidence that Pdzd2 is required for normal regulation of basal insulin secretion. © 2009 Elsevier Ireland Ltd. All rights reserved. ; postprint |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1872-8057 0303-7207 |
| Relation: | Molecular and Cellular Endocrinology; Developmental genomics and skeletal research; Investigating the functional involvement of PDZD2 in the regulation of pancreatic beta cell gene expression and function; http://www.scopus.com/mlt/select.url?eid=2-s2.0-71849102910&selection=ref&src=s&origin=recordpage; Molecular And Cellular Endocrinology, 2010, v. 315 n. 1-2, p. 263-270; 6197372; 270; 169427; WOS:000274608900034; 1-2; http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0303-7207&volume=315&issue=1-2&spage=263&epage=270&date=2010&atitle=Increased+basal+insulin+secretion+in+Pdzd2-deficient+mice; eid_2-s2.0-71849102910; 263; http://hdl.handle.net/10722/68336; 315 |
| DOI: | 10.1016/j.mce.2009.11.007 |
| الاتاحة: | https://doi.org/10.1016/j.mce.2009.11.007 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0303-7207&volume=315&issue=1-2&spage=263&epage=270&date=2010&atitle=Increased+basal+insulin+secretion+in+Pdzd2-deficient+mice http://hdl.handle.net/10722/68336 |
| Rights: | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. ; Molecular and Cellular Endocrinology. Copyright © Elsevier Ireland Ltd. |
| رقم الانضمام: | edsbas.D9B4776B |
| قاعدة البيانات: | BASE |
| تدمد: | 18728057 03037207 |
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| DOI: | 10.1016/j.mce.2009.11.007 |