Academic Journal
Monocyte- and Endothelial-Derived Microparticles Induce an Inflammatory Phenotype in Human Podocytes
| العنوان: | Monocyte- and Endothelial-Derived Microparticles Induce an Inflammatory Phenotype in Human Podocytes |
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| المؤلفون: | Eyre, Jeanette, Burton, James O., Saleem, Moin A., Mathieson, Peter W., Topham, Peter S., Brunskill, Nigel J. |
| المصدر: | Nephron Experimental Nephrology ; volume 119, issue 3, page e58-e66 ; ISSN 1660-2129 |
| بيانات النشر: | S. Karger AG |
| سنة النشر: | 2011 |
| الوصف: | Background/Aims: Proteinuria is associated with cardiovascular and chronic kidney disease. Microparticles (MPs) are bioactive vesicles shed from activated cells and also linked to cardiovascular disease. MP-like structures have been identified in the glomerular basement membrane, urinary space and between the glomerular basement membrane and the podocyte. We hypothesised that circulating MPs may provide a link between vascular injury and kidney diseases by inducing podocyte phenotypic alterations, thus propagating glomerular dysfunction and proteinuria. Methods: Human umbilical vein endothelial cells and U937 monocytes were stimulated with TNF-α to produce MPs. These MPs were confirmed by electron microscopy, and added to differentiated podocyte monolayers to determine effects on podocyte albumin endocytosis and the production of soluble mediators. Results: Monocyte and endothelial MPs upregulated podocyte production of pro-inflammatory mediators monocyte chemoattractant protein-1 (p < 0.001) and interleukin-6 (p < 0.001). Only monocyte MPs upregulated podocyte secretion of VEGF (p < 0.001), known to regulate glomerular permeability. Endothelial MPs decreased podocyte albumin endocytosis by 13% compared to control cells (p < 0.01). Conclusion: MPs alter endocytic functions of podocytes and induce secretion of pro-inflammatory cytokines, potentially leading to glomerular inflammation in vivo and the development of proteinuria. This study identifies a potential pathophysiological role for circulating MPs in the kidney through effects on the podocyte. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1159/000329575 |
| الاتاحة: | http://dx.doi.org/10.1159/000329575 https://www.karger.com/Article/Pdf/329575 |
| Rights: | https://www.karger.com/Services/SiteLicenses ; https://www.karger.com/Services/SiteLicenses |
| رقم الانضمام: | edsbas.D9B3B78A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1159/000329575 |
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