Academic Journal
Identification of Sakurasosaponin as a Cytotoxic Principle from Jacquinia flammea
| العنوان: | Identification of Sakurasosaponin as a Cytotoxic Principle from Jacquinia flammea |
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| المؤلفون: | Sánchez-Medina, Alberto, Peña-Rodríguez, Luis M., May-Pat, Filogonio, Karagianis, Gloria, Waterman, Peter G., Mallet, Anthony I., Habtemariam, Solomon |
| المصدر: | Natural Product Communications ; volume 5, issue 3, page 1934578X1000500 ; ISSN 1934-578X 1555-9475 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2010 |
| الوصف: | The crude ethanolic extract of leaves, stem-bark and roots of J. flammea were tested for their cytotoxic effect against two mammalian cell lines (HeLa and RAW 264.7) and four bacterial species (Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa). When tested at the concentration of 100 μg/mL, the root extract showed the highest cytotoxic activity against mammalian cells followed by the stem-bark extract while the leaves extract did not show significant activity. No antibacterial activity was detected for all extracts when tested up to 500 μg/disc in the disc diffusion assay. The cytotoxic root extract was subjected to fractionation using solvents of ascending polarity: petroleum ether, chloroform, ethylacetate, butanol and water. The water fraction which showed cytotoxic activity was further subjected to routine bioassay-guided fraction to lead to the isolation of sakurasosaponin as the active principle. The recorded IC 50 value for sakurasosaponin was 11.3 ± 1.52 and 3.8 ± 0.25 μM (n=3) against HeLa and RAW 264.7 respectively. The identification of sakurasosaponin was based on analysis of spectroscopic data. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/1934578x1000500304 |
| DOI: | 10.1177/1934578X1000500304 |
| الاتاحة: | http://dx.doi.org/10.1177/1934578x1000500304 http://journals.sagepub.com/doi/pdf/10.1177/1934578X1000500304 |
| Rights: | http://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.D950E1C7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/1934578x1000500304 |
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