Academic Journal
Stage-Specific Germ-Cell Marker Genes Are Expressed in All Mouse Pluripotent Cell Types and Emerge Early during Induced Pluripotency
| العنوان: | Stage-Specific Germ-Cell Marker Genes Are Expressed in All Mouse Pluripotent Cell Types and Emerge Early during Induced Pluripotency |
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| المؤلفون: | Xu, X., Pantakani, Dasaradha Venkata Krishna, Luehrig, Sandra, Tan, Xiaoying, Khromov, Tatjana, Nolte, Jessica, Dressel, Ralf, Zechner, Ulrich, Engel, Wolfgang |
| بيانات النشر: | Public Library Science |
| سنة النشر: | 2011 |
| المجموعة: | Georg-August-Universität Göttingen: GoeScholar |
| الوصف: | Embryonic stem cells (ESCs) generated from the in-vitro culture of blastocyst stage embryos are known as equivalent to blastocyst inner cell mass (ICM) in-vivo. Though several reports have shown the expression of germ cell/pre-meiotic (GC/PrM) markers in ESCs, their functional relevance for the pluripotency and germ line commitment are largely unknown. In the present study, we used mouse as a model system and systematically analyzed the RNA and protein expression of GC/PrM markers in ESCs and found them to be comparable to the expression of cultured pluripotent cells originated from the germ line. Further, siRNA knockdown experiments have demonstrated the parallel maintenance and independence of pluripotent and GC/PrM networks in ESCs. Through chromatin immunoprecipitation experiments, we observed that pluripotent cells exhibit active chromatin states at GC marker genes and a bivalent chromatin structure at PrM marker genes. Moreover, gene expression analysis during the time course of iPS cells generation revealed that the expression of GC markers precedes pluripotency markers. Collectively, through our observations we hypothesize that the chromatin state and the expression of GC/PrM markers might indicate molecular parallels between in-vivo germ cell specification and pluripotent stem cell generation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| ردمك: | 978-0-00-293172-4 0-00-293172-9 |
| تدمد: | 1932-6203 21799849 |
| Relation: | https://resolver.sub.uni-goettingen.de/purl?gro-2/22620; 000293172900028; https://resolver.sub.uni-goettingen.de/purl?gs-1/8198 |
| DOI: | 10.1371/journal.pone.0022413 |
| الاتاحة: | https://resolver.sub.uni-goettingen.de/purl?gro-2/22620 https://resolver.sub.uni-goettingen.de/purl?gs-1/8198 https://doi.org/10.1371/journal.pone.0022413 |
| Rights: | CC BY 2.5 ; https://creativecommons.org/licenses/by/2.5 |
| رقم الانضمام: | edsbas.D8F9708B |
| قاعدة البيانات: | BASE |
Full Text Finder | ردمك: | 9780002931724 0002931729 |
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| تدمد: | 19326203 21799849 |
| DOI: | 10.1371/journal.pone.0022413 |