Academic Journal
Structural changes enable start codon recognition by the eukaryotic translation initiation complex
| العنوان: | Structural changes enable start codon recognition by the eukaryotic translation initiation complex |
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| المؤلفون: | Hussain, Tanweer, Llácer, José Luis, Fernández, Israel S., Muñoz, Antonio, Martín-Marcos, Pilar, Savva, Christos G., Lorsch, Jon R., Hinnebusch, Alan G., Ramakrishnan, V. |
| المساهمون: | Medical Research Council (UK), Agouron Institute, Louis Jeantet Foundation, National Institutes of Health (US), Human Frontier Science Program, Llácer, José Luis |
| بيانات النشر: | Elsevier Cell Press |
| سنة النشر: | 2014 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| الوصف: | 11 páginas, 7 figuras. Dispone de material suplementario en: t http://dx.doi.org/10.1016/j.cell.2014.10.001. ; During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the AUG start codon. Base pairing with AUG is thought to promote isomerization to a more stable conformation (PIN) that arrests scanning and promotes dissociation of eIF1 from the 40S subunit. Here, we present a cryoEM reconstruction of a yeast preinitiation complex at 4.0 Å resolution with initiator tRNA in the PIN state, prior to eIF1 release. The structure reveals stabilization of the codon-anticodon duplex by the N-terminal tail of eIF1A, changes in the structure of eIF1 likely instrumental in its subsequent release, and changes in the conformation of eIF2. The mRNA traverses the entire mRNA cleft and makes connections to the regulatory domain of eIF2?, eIF1A, and ribosomal elements that allow recognition of context nucleotides surrounding the AUG codon. ; This work was funded by grants to V.R. from the UK Medical Research Council (MC_U105184332), Wellcome Trust Senior Investigator award (WT096570), the Agouron Institute and the Jeantet Foundation, from the NIH (GM62128) previously to J.R.L., and from the Human Frontiers in Science Program (RGP-0028/2009) to A.G.H., J.R.L., and V.R., and by the Intramural Research Program of the NIH (A.G.H., P.M.-M., J.R.L., and A.M.) ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0092-8674 1097-4172 |
| Relation: | Publisher's version; http://dx.doi.org/10.1016/j.cell.2014.10.001; No; Cell 159(3):597-607 (2014); http://hdl.handle.net/10261/176160; http://dx.doi.org/10.13039/501100000265; http://dx.doi.org/10.13039/100009344; http://dx.doi.org/10.13039/100000002; http://dx.doi.org/10.13039/100004412 |
| DOI: | 10.1016/j.cell.2014.10.001 |
| DOI: | 10.13039/501100000265 |
| DOI: | 10.13039/100009344 |
| DOI: | 10.13039/100000002 |
| DOI: | 10.13039/100004412 |
| الاتاحة: | http://hdl.handle.net/10261/176160 https://doi.org/10.1016/j.cell.2014.10.001 https://doi.org/10.13039/501100000265 https://doi.org/10.13039/100009344 https://doi.org/10.13039/100000002 https://doi.org/10.13039/100004412 |
| Rights: | open |
| رقم الانضمام: | edsbas.D8F72106 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00928674 10974172 |
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| DOI: | 10.1016/j.cell.2014.10.001 |