Academic Journal
Cell-cycle analyses using thymidine analogues in fission yeast.
| العنوان: | Cell-cycle analyses using thymidine analogues in fission yeast. |
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| المؤلفون: | Silje Anda, Erik Boye, Beata Grallert |
| المصدر: | PLoS ONE, Vol 9, Iss 2, p e88629 (2014) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2014 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Thymidine analogues are powerful tools when studying DNA synthesis including DNA replication, repair and recombination. However, these analogues have been reported to have severe effects on cell-cycle progression and growth, the very processes being investigated in most of these studies. Here, we have analyzed the effects of 5-ethynyl-2'-deoxyuridine (EdU) and 5-Chloro-2'-deoxyuridine (CldU) using fission yeast cells and optimized the labelling procedure. We find that both analogues affect the cell cycle, but that the effects can be mitigated by using the appropriate analogue, short pulses of labelling and low concentrations. In addition, we report sequential labelling of two consecutive S phases using EdU and 5-bromo-2'-deoxyuridine (BrdU). Furthermore, we show that detection of replicative DNA synthesis is much more sensitive than DNA-measurements by flow cytometry. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC3923809?pdf=render; https://doaj.org/toc/1932-6203; https://doaj.org/article/f02b0eb2468142e0aecae825ceef2b4b |
| DOI: | 10.1371/journal.pone.0088629 |
| الاتاحة: | https://doi.org/10.1371/journal.pone.0088629 https://doaj.org/article/f02b0eb2468142e0aecae825ceef2b4b |
| رقم الانضمام: | edsbas.D8DF7361 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0088629 |