Academic Journal
Processing and subcellular localization of the leader papain-like proteinase of Beet yellows closterovirus
| العنوان: | Processing and subcellular localization of the leader papain-like proteinase of Beet yellows closterovirus |
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| المؤلفون: | Zinovkin, Roman A., Erokhina, Tatyana N., Lesemann, Dietrich-Eckhardt, Jelkmann, Wilhelm, Agranovsky, Alexey A. |
| سنة النشر: | 2003 |
| المجموعة: | OpenAgrar (Senat Bundesforschung, Bundesministeriums für Ernährung und Landwirtschaft) |
| مصطلحات موضوعية: | Text, article, ddc:630 |
| الوصف: | ORF 1a of Beet yellows closterovirus (BYV) encodes the domains of the papain-like proteinase (PCP), methyltransferase (MT) and RNA helicase. BYV cDNA inserts encoding the PCP–MT region were cloned in pGEX vectors next to the glutathione S-transferase gene (GST). In a ‘double tag’ construct, the GST–PCP–MT cDNA was flanked by the 39-terminal six histidine triplets. Following expression in E. coli, the fusion proteins were specifically self-cleaved into the GST–PCP and MT fragments. MT-His6 was purified on Ni–NTA agarose and its N-terminal sequence determined by Edman degradation as GVEEEA, thus providing direct evidence for the Gly588/Gly589 bond cleavage. The GST–PCP fragment purified on glutathione S–agarose was used as an immunogen to produce anti-PCP monoclonal antibodies (mAbs). On Western blots of proteins from virus-infected Tetragonia expansa, the mAbs recognized the 66 kDa protein. Immunogold labelling of BYV-infected tissue clearly indicated association of the PCP with the BYV-induced membranous vesicle aggregates, structures related to closterovirus replication. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Journal of General Virology -- J. Gen. Virol. -- JGV -- 0022-1317 -- 1465-2099 -- 219316-4 -- 2007065-2 -- http://jgv.microbiologyresearch.org/content/journal/jgv/ -- http://www.bibliothek.uni-regensburg.de/ezeit/?2007065; https://doi.org/10.1099/vir.0.19151-0; https://www.openagrar.de/receive/openagrar_mods_00063819; https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00032912/2003_0440.pdf |
| DOI: | 10.1099/vir.0.19151-0 |
| الاتاحة: | https://doi.org/10.1099/vir.0.19151-0 https://www.openagrar.de/receive/openagrar_mods_00063819 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00032912/2003_0440.pdf |
| Rights: | only signed in user ; all rights reserved ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.D8BF8DE6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1099/vir.0.19151-0 |
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