التفاصيل البيبلوغرافية
| العنوان: |
MicroRNA-375 Mediated Regulation on Pre-mRNA Processing Factor 3 in Zebrafish Embryos Exposed to Di-(2-ethylhexyl)phthalate at Low Concentrations |
| المؤلفون: |
Junjie Yu (2210050), Ge Huang (263057), Qi Gong (353167), Kai Zhang (102844), Hossam El Din H. Abdelhafez (14285831), Yuting Du (571678), Jiangfeng Guo (767173) |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Genetics, Molecular Biology, Science Policy, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, small noncoding rnas, mrna prediction indicated, many regulatory functions, likely target mrna, induces epigenetic alterations, di -( 2, 3 ′- utr, 375 mediated regulation, zebrafish embryos exposed, 375 whose expression, organisms exposed, 375 mimic, 375 inhibitor, zf4 cells, study aimed, results improved, prpf3 <, oxidative stress, molecular mechanisms, environmental chemicals, disrupting chemical |
| الوصف: |
Di-(2-ethylhexyl)phthalate (DEHP) is an endocrine-disrupting chemical (EDC) that induces epigenetic alterations, apoptosis, and oxidative stress after biological exposure. MicroRNAs (miRNAs) are a class of small noncoding RNAs with many regulatory functions and play a role in organisms exposed to environmental chemicals. miRNA-mRNA prediction indicated that pre-mRNA processing factor 3 ( PRPF3 ) is a likely target mRNA for miR-375 whose expression is altered by DEHP exposure. However, the interrelation between miR-375 and PRPF3 has not yet been confirmed experimentally. This study aimed to investigate the effects of DEHP on miR-375 and PRPF3 in zebrafish. The expression of miR-375 was downregulated, whereas PRPF3 was upregulated at both transcriptional and post-transcriptional levels upon stimulation with DEHP. The interaction between miR-375 and the 3′-untranslated region (3′-UTR) of PRPF3 was confirmed by a dual fluorescent protein assay and a dual luciferase reporter gene assay. The expression of PRPF3 at both transcriptional and post-transcriptional levels was reduced in ZF4 cells when transfected with a miR-375 mimic but increased when transfected with a miR-375 inhibitor. The results improved our understanding of molecular mechanisms of toxicity upon DEHP exposure and presented miR-375 as a potential novel toxicological biomarker for chemical exposure. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/MicroRNA-375_Mediated_Regulation_on_Pre-mRNA_Processing_Factor_3_in_Zebrafish_Embryos_Exposed_to_Di-_2-ethylhexyl_phthalate_at_Low_Concentrations/21760793 |
| DOI: |
10.1021/acs.chemrestox.2c00257.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.chemrestox.2c00257.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.D8B210DF |
| قاعدة البيانات: |
BASE |