Academic Journal
Evaluation of the Host Response in Various Models of Induced Periodontal Disease in Mice
| العنوان: | Evaluation of the Host Response in Various Models of Induced Periodontal Disease in Mice |
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| المؤلفون: | Molon, Rafael Scaf de, Avila, Erica Dorigatti de, Boas Nogueira, Andressa Vilas, Chaves de Souza, Joao Antonio, Avila-Campos, Mario Julio, Andrade, Cleverton Roberto de, Cirelli, Joni Augusto |
| المساهمون: | Universidade Estadual Paulista (UNESP) |
| بيانات النشر: | Amer Acad Periodontology |
| سنة النشر: | 2014 |
| المجموعة: | Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
| مصطلحات موضوعية: | Cytokines, host-parasite interactions, mice, periodontal diseases, periodontitis, Porphyromonas gingivalis |
| الوصف: | National Council for Scientific and Technological Development Process ; CNPq: 133174/2010-7 ; Background: The aim of this study is to characterize and evaluate the host response caused by three different models of experimental periodontitis in mice.Methods: C57BL/6 wild-type female mice were distributed into six experimental groups and sacrificed at 7, 15, and 30 days after the induction of periodontal disease: 1) group C: no treatment control group; 2) group L: periodontal disease induced by ligature; 3) group G-Pg: oral gavage with Porphyromonas gingivalis (Pg); 4) group G-PgFn: oral gavage with Fusobacterium nucleatum + Pg; 5) group I-Pg: heat-killed Pg injected into the palatal mucosa between the molars; and 6) group I-V: phosphatebuffered saline injected into the palatal mucosa. The samples were used to analyze the immune-inflammatory process in the gingival tissue via descriptive histologic and real-time polymerase chain reaction analyses. The alveolar bone loss was evaluated using microcomputed tomography. The data were analyzed using the Kruskal-Wallis test, followed by a post hoc Dunn test and analysis of variance, followed by a Tukey test using a 5% significance level.Results: Only the ligature model displayed significant alveolar bone loss in the initial period (7 days), which was maintained with time. The group injected with heat-killed Pg displayed significant alveolar bone loss starting from day 15, which continued to progress with time (P < 0.05). A significant increase (P < 0.05) in the gene expression of proinflammatory cytokines (interleukin-6 and -1b) and proteins involved in osteoclastogenesis (receptor activator of nuclear factor-kB ligand and osteoprotegerin) was observed in the ligature group on day 7.Conclusion: The ligature and injection of heat-killed Pg models were the most representative of periodontal disease in humans, whereas the oral gavage models were not effective at inducing the disease under the experimental conditions. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 465-477 |
| اللغة: | English |
| تدمد: | 0022-3492 |
| Relation: | Journal of Periodontology; 3.392; 1,408; http://dx.doi.org/10.1902/jop.2013.130225; Journal Of Periodontology. Chicago: Amer Acad Periodontology, v. 85, n. 3, p. 465-477, 2014.; http://hdl.handle.net/11449/112513; WOS:000332532500018; 2402682969776875; 2628593693450121; orcid:0000-0001-7015-7175 |
| DOI: | 10.1902/jop.2013.130225 |
| الاتاحة: | http://hdl.handle.net/11449/112513 https://doi.org/10.1902/jop.2013.130225 |
| Rights: | closedAccess |
| رقم الانضمام: | edsbas.D8A3537E |
| قاعدة البيانات: | BASE |
| تدمد: | 00223492 |
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| DOI: | 10.1902/jop.2013.130225 |