Academic Journal
Transcription Factor-Directed Re-wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming toward trans-Differentiation
| العنوان: | Transcription Factor-Directed Re-wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming toward trans-Differentiation |
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| المؤلفون: | Dall'Agnese A., Caputo L., Nicoletti C., di Iulio J., Schmitt A., Gatto S., Diao Y., Ye Z., Forcato M., Perera R., Bicciato S., Telenti A., Ren B., Puri P. L. |
| المساهمون: | Dall'Agnese, A., Caputo, L., Nicoletti, C., di Iulio, J., Schmitt, A., Gatto, S., Diao, Y., Ye, Z., Forcato, M., Perera, R., Bicciato, S., Telenti, A., Ren, B., Puri, P. L. |
| سنة النشر: | 2019 |
| المجموعة: | Archivio della ricerca dell'Università di Modena e Reggio Emilia (Unimore: IRIS) |
| مصطلحات موضوعية: | cell identity, chromatin organization, insulated neighborhood, looping interaction, MYOD, trans-differentiation |
| الوصف: | MYOD-directed fibroblast trans-differentiation into skeletal muscle provides a unique model to investigate how one transcription factor (TF) reconfigures the three-dimensional chromatin architecture to control gene expression, which is otherwise achieved by the combinatorial activities of multiple TFs. Integrative analysis of genome-wide high-resolution chromatin interactions, MYOD and CTCF DNA-binding profile, and gene expression, revealed that MYOD directs extensive re-wiring of interactions involving cis-regulatory and structural genomic elements, including promoters, enhancers, and insulated neighborhoods (INs). Re-configured INs were hot-spots of differential interactions, whereby MYOD binding to highly constrained sequences at IN boundaries and/or inside INs led to alterations of promoter-enhancer interactions to repress cell-of-origin genes and to activate muscle-specific genes. Functional evidence shows that MYOD-directed re-configuration of chromatin interactions temporally preceded the effect on gene expression and was mediated by direct MYOD-DNA binding. These data illustrate a model whereby a single TF alters multi-loop hubs to drive somatic cell trans-differentiation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/31519520; info:eu-repo/semantics/altIdentifier/wos/WOS:000495886600008; volume:76; issue:3; firstpage:453; lastpage:472.e8; journal:MOLECULAR CELL; http://hdl.handle.net/11380/1188809; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85074250151; www.molecule.org |
| DOI: | 10.1016/j.molcel.2019.07.036 |
| الاتاحة: | http://hdl.handle.net/11380/1188809 https://doi.org/10.1016/j.molcel.2019.07.036 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D868BA8F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.molcel.2019.07.036 |
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