Academic Journal
Luteolin Isolated from Juncus acutus L., a Potential Remedy for Human Coronavirus 229E
| العنوان: | Luteolin Isolated from Juncus acutus L., a Potential Remedy for Human Coronavirus 229E |
|---|---|
| المؤلفون: | Hakem, Asma, Desmarets, Lowiese, Sahli, Ramla, Malek, Rawen Ben, Camuzet, Charline, François, Nathan, Lefèvre, Gabriel, Samaillie, Jennifer, Moureu, Sophie, Sahpaz, Sevser, Belouzard, Sandrine, Ksouri, Riadh, Séron, Karin, Rivière, Céline |
| المساهمون: | BioEcoAgro - UMR transfrontalière INRAE 1158, Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille), Université de Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 1420-3049 ; Molecules ; https://hal.science/hal-04237724 ; Molecules, 2023, 28 (11), pp.4263. ⟨10.3390/molecules28114263⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio], [CHIM]Chemical Sciences |
| الوصف: | International audience ; The COVID-19 pandemic, caused by SARS-CoV-2, addressed the lack of specific antiviral drugs against coronaviruses. In this study, bioguided fractionation performed on both ethyl acetate and aqueous sub-extracts of Juncus acutus stems led to identifying luteolin as a highly active antiviral molecule against human coronavirus HCoV-229E. The apolar sub-extract (CH2Cl2) containing phenanthrene derivatives did not show antiviral activity against this coronavirus. Infection tests on Huh-7 cells, expressing or not the cellular protease TMPRSS2, using luciferase reporter virus HCoV-229E-Luc showed that luteolin exhibited a dose-dependent inhibition of infection. Respective IC50 values of 1.77 µM and 1.95 µM were determined. Under its glycosylated form (luteolin-7-O-glucoside), luteolin was inactive against HCoV-229E. Time of addition assay showed that utmost anti-HCoV-229E activity of luteolin was achieved when added at the post-inoculation step, indicating that luteolin acts as an inhibitor of the replication step of HCoV-229E. Unfortunately, no obvious antiviral activity for luteolin was found against SARS-CoV-2 and MERS-CoV in this study. In conclusion, luteolin isolated from Juncus acutus is a new inhibitor of alphacoronavirus HCoV-229E. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-04237724; https://hal.science/hal-04237724; https://hal.science/hal-04237724/document; https://hal.science/hal-04237724/file/molecules-28-04263-v2.pdf |
| DOI: | 10.3390/molecules28114263 |
| الاتاحة: | https://hal.science/hal-04237724 https://hal.science/hal-04237724/document https://hal.science/hal-04237724/file/molecules-28-04263-v2.pdf https://doi.org/10.3390/molecules28114263 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.D85D7EB0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/molecules28114263 |
|---|