Dissertation/ Thesis
Immunomodulatory Effect of Extracellular Vesicles Secreted by Canine Adipose Tissue Derived Mesenchymal Stem/Stromal Cell in Mouse Models of Inflammatory Bowel Disease
| العنوان: | Immunomodulatory Effect of Extracellular Vesicles Secreted by Canine Adipose Tissue Derived Mesenchymal Stem/Stromal Cell in Mouse Models of Inflammatory Bowel Disease |
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| المؤلفون: | 안주현 |
| المساهمون: | 윤화영, 수의과대학 수의학과 |
| بيانات النشر: | 서울대학교 대학원 |
| سنة النشر: | 2020 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | Dog, Colitis mice, Extracellular Vesicles, M2 macrophage, Regulatory T cells, 개/ 대장염/ 세포 밖 소포체/ M2 대식세포/ 조절 T 세포, 636.089 |
| الوصف: | 학위논문 (박사) -- 서울대학교 대학원 : 수의과대학 수의학과, 2020. 8. 윤화영. ; The inflammatory bowel diseases (IBD) are characterized by relapsing inflammation and immune activation diseases of the gastrointestinal tract. Extracellular vesicles, which elicit similar biological activity to the stem cell themselves, have been used experimentally to treat dextran sulfate sodium (DSS)-induced colitis in murine models though immunosuppressive potential. However, the underlying mechanisms have not been fully elucidated. Tumor necrosis factor (TNF)-α stimulated gene/ protein 6 (TSG-6) secreted from stem cells is a major factor responsible for regulation of inflammatory responses. However, studies on TSG-6 in EVs have not yet been conducted, and, thus, further studies are required. This study aimed to assess therapeutic effects of canine adipose tissue derived mesenchymal stem/stromal cell (cASC)-produced EV in IBD mice models and explore the mechanism underlying the immunomodulatory properties. In first, the role of extracellular vesicles secreted from cASCs was investigated. GW4869, an exosome production inhibitor, was pretreated on stem cells to confirm the role of extracellular vesicles. In the case of stem cells treated with GW4869, it was confirmed that the immunomodulatory ability decreased significantly. Concanavalin A treated canine lymphocytes showed significantly increased level of pro-inflammatory cytokines, such as tumor necrosis factor α (TNF-α), interferon-γ (IFN-γ), interleukin-1β (IL-1 β) and interleukin-6 (IL-6) compared to the naïve group (TNF-α, 27.7 fold, p<0.001; IFN-γ, 125 fold, p<0.001; IL-1 β, 13.9 fold, p<0.001; IL-6, 7.9 fold, p<0.001). Pretreatment with GW4869 significantly reduced the immunosuppressive effects of cASCs (TNF-α, p<0.05; IFN- γ, p<0.05; IL-1β, p<0.005; IL-6, p<0.05). In addition, as a result of co-culturing the extracellular vesicle obtained through repeated ultra-high-speed centrifugation with immune cells, it was confirmed that it has an immunomodulatory effect such as stem ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | English |
| ردمك: | 978-0-00-000162-7 978-0-00-000000-2 0-00-000162-7 0-00-000000-0 |
| Relation: | 000000162767; http://hdl.handle.net/10371/170269; http://dcollection.snu.ac.kr/common/orgView/000000162767; I804:11032-000000162767; 000000000043▲000000000048▲000000162767▲ |
| الاتاحة: | http://hdl.handle.net/10371/170269 http://dcollection.snu.ac.kr/common/orgView/000000162767 |
| رقم الانضمام: | edsbas.D7AB9FA9 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780000001627 9780000000002 0000001627 0000000000 |
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