Academic Journal
From morphological heterogeneity at alveolar level to the overall mechanical lung behavior: an in vivo microscopic imaging study
| العنوان: | From morphological heterogeneity at alveolar level to the overall mechanical lung behavior: an in vivo microscopic imaging study |
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| المؤلفون: | Mazzuca, E, Salito, C, Aliverti, A, Miserocchi, G., RIVOLTA, ILARIA |
| المساهمون: | Mazzuca, E, Salito, C, Rivolta, I, Aliverti, A, Miserocchi, G |
| بيانات النشر: | John Wiley & Sons Ltd GB |
| سنة النشر: | 2014 |
| المجموعة: | Università degli Studi di Milano-Bicocca: BOA (Bicocca Open Archive) |
| مصطلحات موضوعية: | Alveolar mechanic, in vivo microscopy, multiscale |
| الوصف: | In six male anesthetized, tracheotomized, and mechanically ventilated rabbits, we imaged subpleural alveoli under microscopic view (60*) through a "pleural window" obtained by stripping the endothoracic fascia and leaving the parietal pleura intact. Three different imaging scale levels were identified for the analysis on increasing stepwise local distending pressure (P ld) up to 16.5 cmH2O: alveoli, alveolar cluster, and whole image field. Alveolar profiles were manually traced, clusters of alveoli of similar size were identified through a contiguity-constrained hierarchical agglomerative clustering analysis and alveolar surface density (ASD) was estimated as the percentage of air on the whole image field. Alveolar area distributions were remarkably right-skewed and showed an increase in median value with a large topology-independent heterogeneity on increasing P ld. Modeling of alveolar area distributions on increasing P ld led to hypothesize that absolute alveolar compliance (change in surface area over change in P ld) increases fairly linearly with increasing initial alveolar size, the corollary of this assumption being a constant specific compliance. Clusters were reciprocally interweaved due to their highly variable complex shapes. ASD was found to increase with a small coefficient of variation (CV <25%) with increasing P ld. The CV of lung volume at each transpulmonary pressure was further decreased (about 6%). The results of the study suggest that the considerable heterogeneity of alveolar size and of the corresponding alveolar mechanical behavior are homogenously distributed, resulting in a substantially homogenous mechanical behavior of lung units and whole organ |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/24744890; info:eu-repo/semantics/altIdentifier/wos/NLM Unique ID:101607800; volume:2; issue:2; journal:PHYSIOLOGICAL REPORTS; http://hdl.handle.net/10281/100360 |
| DOI: | 10.1002/phy2.221 |
| الاتاحة: | http://hdl.handle.net/10281/100360 https://doi.org/10.1002/phy2.221 |
| رقم الانضمام: | edsbas.D79A5885 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/phy2.221 |
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