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Quantification of CCR5 mRNA in Human Lymphocytes and Macrophages by Real-Time Reverse Transcriptase PCR Assay

التفاصيل البيبلوغرافية
العنوان: Quantification of CCR5 mRNA in Human Lymphocytes and Macrophages by Real-Time Reverse Transcriptase PCR Assay
المؤلفون: Lai, Jian-Ping, Yang, Ji-Hong, Douglas, Steven D., Wang, Xu, Riedel, Eric, Ho, Wen-Zhe
المصدر: Clinical and Vaccine Immunology ; volume 10, issue 6, page 1123-1128 ; ISSN 1556-6811 1556-679X
بيانات النشر: American Society for Microbiology
سنة النشر: 2003
الوصف: CCR5, a β-chemokine receptor, plays an important role in human immunodeficiency virus (HIV) infection of human immune cells, as it is a primary coreceptor for HIV entry into macrophages. We have applied a newly developed real-time reverse transcriptase PCR (RT-PCR) assay for the quantification of CCR5 mRNA in human blood immune cells. The CCR5 real-time RT-PCR assay has a sensitivity of 100 mRNA copies, with a dynamic range of detection between 10 2 and 10 6 copies of the CCR5 mRNA transcripts per reaction. The assay is highly reproducible, with an intra-assay coefficient of variation of the threshold cycle of less than 2.07%. When used for quantification of CCR5 mRNA in human monocyte-derived macrophages (MDM) and peripheral blood lymphocytes (PBL), the assay has precision and reproducibility. MDM expressed higher levels of CCR5 mRNA than did PBL. Thus, this assay has the potential and a wide application for the investigation of the role of CCR5 in inflammatory diseases and viral infections, including HIV disease.
نوع الوثيقة: article in journal/newspaper
اللغة: English
DOI: 10.1128/cdli.10.6.1123-1128.2003
DOI: 10.1128/CDLI.10.6.1123-1128.2003
الاتاحة: http://dx.doi.org/10.1128/cdli.10.6.1123-1128.2003
https://journals.asm.org/doi/pdf/10.1128/CDLI.10.6.1123-1128.2003
Rights: https://journals.asm.org/non-commercial-tdm-license
رقم الانضمام: edsbas.D71CE3F8
قاعدة البيانات: BASE
الوصف
DOI:10.1128/cdli.10.6.1123-1128.2003