Academic Journal
A partially assembled complex I in NAD4‐deficient mitochondria of maize
| العنوان: | A partially assembled complex I in NAD4‐deficient mitochondria of maize |
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| المؤلفون: | Karpova, Olga V., Newton, Kathleen J. |
| المصدر: | The Plant Journal ; volume 17, issue 5, page 511-521 ; ISSN 0960-7412 1365-313X |
| بيانات النشر: | Wiley |
| سنة النشر: | 1999 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Summary The proton‐translocating NADH:ubiquinone oxidoreductase (respiratory complex I) consists of at least 32 subunits in higher plants, nine of which are mitochondrially encoded (NAD 1–7, NAD4L, NAD9). Complex I (CI) has been analyzed from a mitochondrial mutant of maize, NCS2, that carries a deletion for the 3′ end of the nad4 gene. Mitochondria from highly defective, near‐homoplasmic mutant plants have only trace amounts of the normal complex I. Instead, a reduced amount of a smaller complex, which also exhibits NADH dehydrogenase activity, is detected on ‘blue‐native’ polyacrylamide gels. Subunits of 76 kDa, 40 kDa and 55 kDa, as well as NAD7 and NAD9, have been identified in the subcomplex by their cross‐reactivity with heterologous antisera. The corresponding subunits in Neurospora are localized in a ‘peripheral arm’ of CI, which is known to assemble independently of a ‘membrane arm’. The maize NCS2 CI subcomplex is loosely bound to the membrane and is missing several subunits that could be membrane components. Thus, the mutant CI subcomplex may consist of a peripheral arm. A reduction in the steady‐state levels of NAD7 and NAD9 in NCS2 mitochondria occurs despite normal rates of biosynthesis and there is a concomitant decrease of the nuclear encoded 76 kDa subunit. The reduction in CI‐associated NADH dehydrogenase activity in the nad4 ‐deficient NCS2 mutant mitochondria is not associated with a compensatory increase in the activities or amounts of the putative ‘exogenous’ NAD(P)H dehydrogenases that are found in plant mitochondria. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1046/j.1365-313x.1999.00401.x |
| DOI: | 10.1046/j.1365-313X.1999.00401.x |
| الاتاحة: | http://dx.doi.org/10.1046/j.1365-313x.1999.00401.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-313X.1999.00401.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-313X.1999.00401.x https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-313X.1999.00401.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.D715F112 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1046/j.1365-313x.1999.00401.x |
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