التفاصيل البيبلوغرافية
| العنوان: |
BODIPY derivatives: design, synthesis, and evaluation as chemosensors, probes for bioimaging, and photosensitizers for PDT ; Derivados de BODIPY: design, síntese e avaliação como sensores químicos, sondas para bioimagem e fotossensibilizadores para PDT |
| المؤلفون: |
Pinto, Sónia Catarina Silva |
| المساهمون: |
Raposo, M. Manuela M., Pina, João Manuel Ferreira Pita Batista |
| سنة النشر: |
2022 |
| المجموعة: |
Universidade of Minho: RepositóriUM |
| مصطلحات موضوعية: |
AIE Fluorophores, Bioimaging, BODIPY, Optical chemosensors, Photodynamic therapy, Bioimagem, Fluoróforos AIE, Sensores óticos, Terapia fotodinâmica, Ciências Naturais::Outras Ciências Naturais |
| الوصف: |
Dissertação de mestrado em Técnicas de Caracterização e Análise Química ; 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene derivatives, commonly referred as BODIPYs, constitute a class of fluorophores that has been arousing great curiosity in the scientific community. The excellent photophysical properties, the accessible synthesis, and the chemical versatility of BODIPY derivatives explain the growing interest in this class of compounds in recent years, as well as its demand for application in the most diverse areas of research. Thus, this dissertation was based on several objectives: the design, synthesis, and photophysical characterization of BODIPY derivatives functionalized with distinct groups at the meso position of the BODIPY core and the evaluation of the capacity of these compounds for different applications, namely, as optical chemosensors, fluorescent probes for bioimaging, aggregation-induced emission (AIE) fluorophores and photosensitizers for photodynamic therapy. In order to evaluate the application of these derivatives as optical chemosensors, spectrofluorimetric titrations were carried out in the presence of Pd2+, Hg2+, Fe3+, and Al3+, since the preliminary study of sensing capacity revealed a selectivity for these cations. On the other hand, the potential application as a fluorescent probe in bioimaging was evaluated in HeLa cancer cells using fluorescence techniques and confocal microscopy, with promising results. In the evaluation as a AIE fluorophore, the influence of solvent polarity on the spectroscopic and photophysical properties of one of the BODIPY derivatives under study and its emissive behavior in the aggregation state in an aqueous medium was also studied. Finally, for potential application as a photosensitizer for photodynamic therapy, the sensitization of singlet oxygen in solution was evaluated by spectroscopy, and the internalization and cytotoxicity performance of one BODIPY derivative was studied in 4T1 cancer cells using fluorescence techniques and flow cytometry, giving ... |
| نوع الوثيقة: |
master thesis |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FQUI-COL%2F28052%2F2017/PT; https://hdl.handle.net/1822/81274; 203052811 |
| الاتاحة: |
https://hdl.handle.net/1822/81274 |
| Rights: |
info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.D6FAAD99 |
| قاعدة البيانات: |
BASE |