Academic Journal
Performance characteristics of UV and visible spectrophotometry methods for quantitative determination norfloxacin in tablets
| العنوان: | Performance characteristics of UV and visible spectrophotometry methods for quantitative determination norfloxacin in tablets |
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| المؤلفون: | Chierentin, Lucas, Salgado, Hérida Regina Nunes |
| المساهمون: | Universidade Estadual Paulista (UNESP) |
| سنة النشر: | 2014 |
| المجموعة: | Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
| مصطلحات موضوعية: | Norfloxacin, Spectrophotometric methods, Validation, Method Development |
| الوصف: | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) ; PADDC-FCF, FUNDUNESP ; Processo FAPESP: 2010/13335-2 ; This work has proposed the development and validation of ultraviolet (UV) and visible (Vis) spectrophotometric methods for the determination of norfloxacin in the tablets. The proposed methods were applied to pharmaceutical formulation and percent amount of drug estimated (96.08% for UV method and 102.65% for Vis method) and was found in good agreement with the label claim. Using the UV method norfloxacin showed an absorption maximum at 277 nm, in 0.1 M hydrochloridric acid medium, whereas for the Vis spectrophotometric method it reacts with chloranilic acid reagent, forming a purple solution with an absorption maximum at 520 nm. The calibrations curves were linear over the working range of 2.0-7.0 μg.mL-1 for the UV method and 90.0-120.0 μg/mL for the Vis method. The linear regression equation for UV method was y = 0.1303x+0.0026 (r 2 =0.9999) and for Vis method y = 0.0037x-0.0069 (r 2 = 0.9948), they proved to be linear. The methods were completely validated according to the International Conference Harmonization (ICH) guidelines, showing good accuracy, precision, selectivity, linearity and robustness. Therefore the both methods were found to be simple, rapid, sensitive, and easily contributing to the quality control of norfloxacin tablets while being interchangeable. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 531-541 |
| اللغة: | English |
| تدمد: | 2070-0245 |
| Relation: | Journal of Scientific Research; http://dx.doi.org/10.3329/jsr.v6i3.18381; Journal of Scientific Research, v. 6, n. 3, p. 531-541, 2014.; http://hdl.handle.net/11449/133797; 5378339789000363; 9881720291571774 |
| DOI: | 10.3329/jsr.v6i3.18381 |
| الاتاحة: | http://hdl.handle.net/11449/133797 https://doi.org/10.3329/jsr.v6i3.18381 |
| Rights: | closedAccess |
| رقم الانضمام: | edsbas.D6EFFD04 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20700245 |
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| DOI: | 10.3329/jsr.v6i3.18381 |