Academic Journal
Portable sensing system based on electrochemical impedance spectroscopy for the simultaneous quantification of free and total microcystin-LR in freshwaters
| العنوان: | Portable sensing system based on electrochemical impedance spectroscopy for the simultaneous quantification of free and total microcystin-LR in freshwaters |
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| المؤلفون: | Barreiros dos Santos, M, Queirós, R.B, Geraldes, Á, Marques, C, Vilas-Boas, V, Dieguez, L, Paz, E, Ferreira, R, Morais, J, Vasconcelos, V, Piteira, J, Freitas, P.P, Espiña, B. |
| المساهمون: | Centro Interdisciplinar de Investigação Marinha e Ambiental |
| بيانات النشر: | Biosensors & Bioelectronics |
| سنة النشر: | 2019 |
| المجموعة: | Repositório Aberto da Universidade do Porto |
| مصطلحات موضوعية: | Electrochemical impedance spectroscopy, Microcystin-LR, Portable system, Sample processing, Simultaneous detection |
| الوصف: | Microcystins are the most worldwide extended and common toxins produced by cyanobacteria in freshwater. Microcystin-leucine arginine (MC-LR), associated with the most toxic incidents involving microcystins, are within the cyanobacteria (intracellular) until released into the surrounding waters (extracellular) during cell lysis. Therefore, the relationship between intracellular and extracellular cyanotoxins will allow a comprehensive risk of cyanobacteria-containing waters, preventing disease and improving human safety. In this work, we present the development of a novel portable microfluidic sensing platform for the simultaneous detection of free (extracellular) and total MC-LR (intracellular and extracellular). The integrated system contains the sample processing and detection modules capable of performing the chemical lysis, filtration, sample mixing with antibodies, and electrochemical detection of MC-LR based on an indirect strategy. The performance of the immunosensors was evaluated by electrochemical impedance spectroscopy, showing a linear dynamic range between 3.3 × 10−4 and 10−7 g L−1 and a limit of detection of 5.7 × 10−10 g L−1. The results demonstrate the potential of the developed portable biosensor platform and its suitable application for the analysis of MC-LR at regulated levels for drinking water. Finally, the integrated system was able to simultaneously detect the free and total MC-LR on a Microcystis aeruginosa culture. To the best of our knowledge this is the first described system that can differentiate between intracellular and extracellular concentration of MC-LR. This novel electrochemical sensing platform avoids the multiple processing steps typically needed for standard MC-LR analysis in the laboratory and provides an early warning system for MC-LR remote monitoring in water. © 2019 Elsevier B.V. ; The authors acknowledge Ana Castanheira and Marisa P. Sarria for the maintenance of the Microcystis aeruginosa cultures and Laura Rodriguez-Lorenzo for helping in SEM characterisation. This ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0956-5663 |
| Relation: | Biosensors and Bioelectronics 142 (2019) 111550; https://www.sciencedirect.com/science/article/pii/S0956566319306293; https://hdl.handle.net/10216/130505; https://doi.org/10.1016/j.bios.2019.111550 |
| DOI: | 10.1016/j.bios.2019.111550 |
| الاتاحة: | https://hdl.handle.net/10216/130505 https://doi.org/10.1016/j.bios.2019.111550 |
| Rights: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.D6DC1C3B |
| قاعدة البيانات: | BASE |
| تدمد: | 09565663 |
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| DOI: | 10.1016/j.bios.2019.111550 |