Academic Journal
POC device for rapid oral pH determination based on a smartphone platform
| العنوان: | POC device for rapid oral pH determination based on a smartphone platform |
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| المؤلفون: | Arroyo, Manuel J., Escobedo Araque, Pablo, Ruiz García, Isidoro, Palma López, Alberto José, Santoyo González, Francisco, Ortega Muñoz, Mariano, Capitán Vallvey, Luis Fermín, Erenas Rodríguez, Miguel María |
| سنة النشر: | 2024 |
| المجموعة: | DIGIBUG: Repositorio Institucional de la Universidad de Granada |
| مصطلحات موضوعية: | Colourimetric sensors, Smartphone color detection, Salivary pH determination, Anthocyanins, Poin-of-Care (POC) |
| الوصف: | Salivary pH serves as a valuable and useful diagnostic marker for periodontal disease, as it not only plays a critical role in disease prevention but also in its development. Typically, saliva sampling is collected by draining and spitting it into collec- tion tubes or using swabs. In this study, we have developed a Point-of-Care (POC) device for in situ determination of oral pH without the need for complex instruments, relying solely on a smartphone as the detection device. Our system utilizes a non-toxic vegetable colourimetric indicator, immobilized on a chitosan membrane located on a disposable stick, enabling direct sampling within the buccal cavity. An ad hoc designed 3D-printed attachment is used to ensure accurate positioning and alignment of the stick, as well as isolation from external lighting conditions. A custom-developed smartphone application captures and automatically processes the image of the sensing membrane, providing the salivary pH results. After optimiz- ing the cocktail composition, the developed sensors demonstrated the capacity to determine pH within a range of 5.4 to 8.1 with a remarkable precision of 0.6%, achieving a very short analysis time of just 1 min. A stability study conducted on the sensing membranes revealed a lifetime of 50 days. To validate the performance of our analytical device, we compared its results against those obtained from a calibrated pH-meter, using a group of individuals. The device exhibited an average error of 2.4% when compared with the pH-meter results, confirming its reliability and accuracy ; Funding for open access publishing: Universidad de Gra- nada/CBUA. This work was funded by Spanish MCIN (Project PID2019-103938RB-I00, Project PID2022-138727OB-I00 funded by MCIN/AEI/10.13039/501100011033 and, by ERDF “A way of making Europe”, by the “European Union” and grant IJC2020- 043307-I) and Junta de Andalucía (Projects B-FQM-243-UGR18, P18-RT-2961). The projects and the grant were partially supported by European Regional Development Funds (ERDF) and by ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Microchim Acta 191, 134 (2024); https://hdl.handle.net/10481/89519; https://doi.org/10.1007/s00604-024-06227-1 |
| DOI: | 10.1007/s00604-024-06227-1 |
| الاتاحة: | https://hdl.handle.net/10481/89519 https://doi.org/10.1007/s00604-024-06227-1 |
| Rights: | Atribución-NoComercial 4.0 Internacional ; http://creativecommons.org/licenses/by-nc/4.0/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D6B78861 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00604-024-06227-1 |
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