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Identification and characterization of C N67A +M F37L revertant.
| العنوان: | Identification and characterization of C N67A +M F37L revertant. |
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| المؤلفون: | Anastazia Jablunovsky, Anoop Narayanan, Joyce Jose |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Microbiology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Cancer, Infectious Diseases, Virology, Computational Biology, persistent health concerns, partially restored phenotype, molecular interactions involved, infectious cdna clone, induced diseases require, including genome packaging, highly attenuated phenotype, first experimental evidence, exposed amino acids, endemic regions worldwide, alanine scanning mutagenesis, vero e6 cells, div >< p, c &# 945, viral envelope protein, second site mutation, >- transmitted flaviviruses, zikv virion production, purified c protein, flavivirus capsid protein, c α3 revealed |
| الوصف: | (A) Micrographs showing fluorescent clusters of Vero E6 cells infected with ZIKV venus -C N67A at passage 0 (P0) and passage 5 (P5). (B) Sanger sequencing results analyzed using FinchTV software showing the region of mutation in M protein in the P5 revertant virus. A single nucleotide T→C mutation (highlighted in blue) created an F→L amino acid mutation in the M protein resulting in a ZIKV venus -C N67A+ +M F37L double mutant. (C) Virus titers of ZIKV venus -C N67A mutant and ZIKV venus -C N67A+ +M F37L revertant compared to wild-type ZIKV venus as determined by plaque assay on Vero E6 cells. The graph represents average titers (n = 3) with error bars representing SEM. Statistical significance was calculated by Ordinary one-way ANOVA using GraphPad PRISM 7 software at a 95% confidence interval. Relative significance is indicated by asterisks (p<0.01 = **). (D) Growth curve analysis of ZIKV WT and ZIKV WT -C N67A+ +M F37L . The graph represents average titers (n = 3) at 24-, 48-, 72-, and 96 h.p.i with error bars representing SEM. (E) Western blot of virus pellets obtained after ultracentrifugation of the cell culture supernatants probed with anti-ZIKV C antibody. Label -C on the right indicates a band corresponding to the released ZIKV C protein. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/Identification_and_characterization_of_C_sub_N67A_sub_M_sub_F37L_sub_revertant_/24931976 |
| DOI: | 10.1371/journal.pntd.0011873.g006 |
| الاتاحة: | https://doi.org/10.1371/journal.pntd.0011873.g006 https://figshare.com/articles/figure/Identification_and_characterization_of_C_sub_N67A_sub_M_sub_F37L_sub_revertant_/24931976 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.D6AC1F4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pntd.0011873.g006 |
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