Academic Journal
Purification, Partial Characterizations, and N‐Terminal Amino Acid Sequence of a Procoagulant Protein FV‐2 from Daboia Russelli Siamensis (Myanmar) Venom
| العنوان: | Purification, Partial Characterizations, and N‐Terminal Amino Acid Sequence of a Procoagulant Protein FV‐2 from Daboia Russelli Siamensis (Myanmar) Venom |
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| المؤلفون: | Zhu, Weiwei, Wu, Zheng, Shen, Shuhao, Liu, Jun, Xiang, Nanlin, Liao, Yunjian, Lin, Xi, Chen, Lixin, Chen, Qi |
| المساهمون: | Department of Health of Guangdong Province |
| المصدر: | Journal of Biochemical and Molecular Toxicology ; volume 29, issue 10, page 465-471 ; ISSN 1095-6670 1099-0461 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2015 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | In this study, we purified and characterized the procoagulant protein FV‐2 from Daboia russelli siamensis (Myanmar) venom using ion‐exchange chromatography on CM‐Sephadex C‐50 and gel filtration on Superdex TM G‐75 column. The activation of factor X and prothrombin was determined, respectively, by specific chromogenic substrates. The fibrinogen‐clotting activity, thermal stability, and pH stability were also determined. The N‐treminal sequence was determined by the National Center of Biomedical Analysis of China. In the end, FV‐2 was achieved with a molecular weight of 13,608.0 Da. It could activate factor X, but did not affect prothrombin or fibrinogen. The suitable pH was 6.5–7.5, and the suitable temperature ranged from 25 to 60°C. The N‐terminal sequence was Asn‐Phe‐Phe‐Gln‐Phe‐Ala‐Glu‐Met‐Ile‐Val‐Lys‐Met‐Thr‐Gly‐Lys. Taken together, our studies suggest that FV‐2 is a factor X–activating enzyme, which can activate factor X to factor Xa, but it has no effect on prothrombin and fibrinogen. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/jbt.21713 |
| الاتاحة: | http://dx.doi.org/10.1002/jbt.21713 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjbt.21713 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jbt.21713 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.D69A7094 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/jbt.21713 |
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