Isolation of chromatography fractions from TZSV-infected plants and in vitro cleavage assay of fractions using TZSV S RNA.

التفاصيل البيبلوغرافية
العنوان: Isolation of chromatography fractions from TZSV-infected plants and in vitro cleavage assay of fractions using TZSV S RNA.
المؤلفون: Hao Hong (337751), Chunli Wang (615437), Ying Huang (53474), Min Xu (15203), Jiaoling Yan (11198601), Mingfeng Feng (3411317), Jia Li (160557), Yajie Shi (6255656), Min Zhu (168912), Danyu Shen (113434), Peijun Wu (6740249), Richard Kormelink (182162), Xiaorong Tao (849312)
سنة النشر: 2021
المجموعة: Smithsonian Institution: Digital Repository
مصطلحات موضوعية: Biochemistry, Medicine, Microbiology, Cell Biology, Molecular Biology, Cancer, Mental Health, Infectious Diseases, Plant Biology, Virology, Biological Sciences not elsewhere classified, genomic RNA strands, genomic RNAs complexed, Gn gene fragments, genomic RNA, planta RISC complexes, TRV, subgenomic mRNA molecules, tobacco rattle virus, 2 b gene fragments, targets tospoviral mRNAs whilst, tospovirus Antiviral RNA, CMV genomic RNA, RNP, NSV tospoviral genomic RNA, tospoviral genomic RNAs
الوصف: Sixteen Superdex S-200 fractions were isolated from TZSV infected N . benthamiana . Every fraction (600 μL each) was incubated in vitro with digoxigenin (DIG) labeled full length S RNA of TZSV (100 ng) to examine which fraction contained the RISC associated nuclease activity to viral S RNA. (TIF)
نوع الوثيقة: still image
اللغة: unknown
Relation: https://figshare.com/articles/figure/Isolation_of_chromatography_fractions_from_TZSV-infected_plants_and_i_in_vitro_i_cleavage_assay_of_fractions_using_TZSV_S_RNA_/15069522
DOI: 10.1371/journal.ppat.1009757.s005
الاتاحة: https://doi.org/10.1371/journal.ppat.1009757.s005
Rights: CC BY 4.0
رقم الانضمام: edsbas.D693D68E
قاعدة البيانات: BASE
الوصف
DOI:10.1371/journal.ppat.1009757.s005