Academic Journal
Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology
| العنوان: | Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology |
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| المؤلفون: | James M Dominguez, Sharon M Moe, Neal X Chen, Todd O McKinley, Krista M Brown, Yunlong Liu, Hongyu Gao, Roman M Natoli |
| المصدر: | BioTechniques, Vol 71, Iss 2, Pp 431-438 (2021) |
| بيانات النشر: | Taylor & Francis Group |
| سنة النشر: | 2021 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | cell isolation, cryopreservation, fracture, nonunion, single-cell RNA sequencing, Biology (General), QH301-705.5 |
| الوصف: | The ability to study the bone microenvironment of failed fracture healing may lead to biomarkers for fracture nonunion. Herein the authors describe a technique for isolating individual cells suitable for single-cell RNA sequencing analyses from intramedullary canal tissue collected by reaming during surgery. The purpose was to detail challenges and solutions inherent to the collection and processing of intramedullary canal tissue samples. The authors then examined single-cell RNA sequencing data from fresh and reanimated samples to demonstrate the feasibility of this approach for prospective studies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818; https://doaj.org/article/cdd5b915484a40638d1700d57d37cbf7 |
| DOI: | 10.2144/btn-2021-0002 |
| الاتاحة: | https://doi.org/10.2144/btn-2021-0002 https://doaj.org/article/cdd5b915484a40638d1700d57d37cbf7 |
| رقم الانضمام: | edsbas.D6840288 |
| قاعدة البيانات: | BASE |
| DOI: | 10.2144/btn-2021-0002 |
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