Academic Journal
Signaling via Interleukin-4 Receptor α Chain Is Required for Successful Vaccination against Schistosomiasis in BALB/c Mice
| العنوان: | Signaling via Interleukin-4 Receptor α Chain Is Required for Successful Vaccination against Schistosomiasis in BALB/c Mice |
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| المؤلفون: | Mountford, Adrian P., Hogg, Karen G., Coulson, Patricia S., Brombacher, Frank |
| المساهمون: | Petri, W. A. |
| المصدر: | Infection and Immunity ; volume 69, issue 1, page 228-236 ; ISSN 0019-9567 1098-5522 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2001 |
| الوصف: | Although protective immunity in C57BL/6 mice induced by a single dose of the radiation-attenuated schistosome vaccine is believed to be mediated by Th1-type immune responses, we here report that in BALB/c mice protection can also depend upon signaling via the interleukin-4 (IL-4) receptor which conventionally governs the development of Th2-type immune responses. We show that in BALB/c mice deficient for the IL-4 receptor α chain (IL-4Rα −/− ), which are unresponsive to IL-4 and IL-13, vaccine-induced protection is abrogated compared with that in wild-type (WT) mice. In vaccinated IL-4Rα −/− mice, IL-12p40 production by cells from the skin exposure site was elevated, although gamma interferon (IFN-γ) production in draining lymphoid tissues was similar in WT and IL-4Rα −/− mice. Nevertheless, the effector response in IL-4Rα −/− mice was Th1 biased with elevated IFN-γ in the lungs and higher immunoglobulin G2a (IgG2a) and IgG2b titers but negligible quantities of Th2-associated IgG1 and IgE. Interestingly, levels of IL-4 were equivalent in WT and IL-4Rα −/− mice, indicating that Th2 responses were not dependent upon signaling by IL-4 or IL-13. No differences in the phenotype and composition of the pulmonary effector mechanism that might explain the failure to induce protection in IL-4Rα −/− mice were detected. However, passive transfer of partial protection to naive IL-4Rα −/− mice, using serum from vaccinated WT mice, indicates that Th2-associated antibodies such as IgG1 have a role in parasite elimination in BALB/c strain mice and that signaling via IL-4R can be an important factor in the generation of protection. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/iai.69.1.228-236.2001 |
| DOI: | 10.1128/IAI.69.1.228-236.2001 |
| الاتاحة: | http://dx.doi.org/10.1128/iai.69.1.228-236.2001 https://journals.asm.org/doi/pdf/10.1128/IAI.69.1.228-236.2001 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.D66D4F9D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/iai.69.1.228-236.2001 |
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