Academic Journal
Shape-defined solid micro-objects from poly(d,l-lactic acid) as cell-supportive counterparts in bottom-up tissue engineering
| العنوان: | Shape-defined solid micro-objects from poly(d,l-lactic acid) as cell-supportive counterparts in bottom-up tissue engineering |
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| المؤلفون: | Leferink, A.M., Tibbe, M.P., Bossink, E.G.B.M., de Heus, L.E., van Vossen, H., van den Berg, A., Moroni, L., Truckenmüller, R.K. |
| المصدر: | Leferink , A M , Tibbe , M P , Bossink , E G B M , de Heus , L E , van Vossen , H , van den Berg , A , Moroni , L & Truckenmüller , R K 2019 , ' Shape-defined solid micro-objects from poly(d,l-lactic acid) as cell-supportive counterparts in bottom-up tissue engineering ' , Materials today. Bio , vol. 4 , 100025 . https://doi.org/10.1016/j.mtbio.2019.100025 |
| سنة النشر: | 2019 |
| المجموعة: | Maastricht University Research Publications |
| مصطلحات موضوعية: | Poly(lactic acid), Hot embossing/thermal imprinting, Human bone marrow stromal cells, Human umbilical vein endothelial cells, Bone tissue engineering, Self-assembly, NANOIMPRINT LITHOGRAPHY, PROLIFERATION, APOPTOSIS, COMPOSITE, HYDROGELS, FUSION |
| الوصف: | In bottom-up tissue engineering, small modular units of cells and biomaterials are assembled toward larger and more complex ones. In conjunction with a new implementation of this approach, a novel method to fabricate microscale objects from biopolymers by thermal imprinting on water-soluble sacrificial layers is presented. By this means, geometrically well-defined objects could be obtained without involving toxic agents in the form of photoinitiators. The micro-objects were used as cell-adhesive substrates and cell spacers in engineered tissues created by cell-guided assembly of the objects. Such constructs can be applied both for in vitro studies and clinical treatments. Clinically relevantly sized aggregates comprised of cells and micro-objects retained their viability up to 2 weeks of culture. The aggregation behavior of cells and objects showed to depend on the type and number of cells applied. To demonstrate the micro-objects' potential for engineering vascularized tissues, small aggregates of human bone marrow stromal cells (hMSCs) and micro-objects were coated with a layer of human umbilical vein endothelial cells (HUVECs) and fused into larger tissue constructs, resulting in HUVEC-rich regions at the aggregates' interfaces. This three-dimensional network-type spatial cellular organization could foster the establishment of (premature) vascular structures as a vital prerequisite of, for example, bottom-up-engineered bone-like tissue. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.mtbio.2019.100025 |
| الاتاحة: | https://cris.maastrichtuniversity.nl/en/publications/4e1bcbe0-f366-449d-9c6c-5be28ce4fda8 https://doi.org/10.1016/j.mtbio.2019.100025 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D60FB469 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.mtbio.2019.100025 |
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