Academic Journal
Exploiting protein n-terminus for site-specific bioconjugation
| العنوان: | Exploiting protein n-terminus for site-specific bioconjugation |
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| المؤلفون: | De Rosa L., Di Stasi R., Romanelli A., D'andrea L. D. |
| المساهمون: | L. De Rosa, R. Di Stasi, A. Romanelli, L.D. D'andrea |
| بيانات النشر: | MDPI |
| سنة النشر: | 2021 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | aldehyde protein, azide protein, chemical ligation, chemo-selective reaction, click-chemistry, molecular probe, protein labeling, Settore CHIM/03 - Chimica Generale e Inorganica, Settore CHIM/06 - Chimica Organica |
| الوصف: | Although a plethora of chemistries have been developed to selectively decorate protein molecules, novel strategies continue to be reported with the final aim of improving selectivity and mildness of the reaction conditions, preserve protein integrity, and fulfill all the increasing require-ments of the modern applications of protein conjugates. The targeting of the protein N-terminal alpha-amine group appears a convenient solution to the issue, emerging as a useful and unique reactive site universally present in each protein molecule. Herein, we provide an updated overview of the methodologies developed until today to afford the selective modification of proteins through the targeting of the N-terminal alpha-amine. Chemical and enzymatic strategies enabling the selective labeling of the protein N-terminal alpha-amine group are described. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/34207845; info:eu-repo/semantics/altIdentifier/wos/WOS:000667437800001; volume:26; issue:12; firstpage:1; lastpage:20; numberofpages:20; journal:MOLECULES; http://hdl.handle.net/2434/859856; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85108543985 |
| DOI: | 10.3390/molecules26123521 |
| الاتاحة: | http://hdl.handle.net/2434/859856 https://doi.org/10.3390/molecules26123521 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D60EE723 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/molecules26123521 |
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