Academic Journal
RNAi knock‐downs support roles for the mucin‐like ( AeIMUC1) gene and short‐chain dehydrogenase/reductase ( SDR) gene in Aedes aegypti susceptibility to Plasmodium gallinaceum
| العنوان: | RNAi knock‐downs support roles for the mucin‐like ( AeIMUC1) gene and short‐chain dehydrogenase/reductase ( SDR) gene in Aedes aegypti susceptibility to Plasmodium gallinaceum |
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| المؤلفون: | BEROIS, M., ROMERO‐SEVERSON, J., SEVERSON, D. W. |
| المصدر: | Medical and Veterinary Entomology ; volume 26, issue 1, page 112-115 ; ISSN 0269-283X 1365-2915 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2011 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | The mosquito midgut represents the first barrier encountered by the Plasmodium parasite (Haemosporida: Plasmodiidae) when it is ingested in blood from an infected vertebrate. Previous studies identified the Aedes aegypti (L.) (Diptera: Culicidae) mucin‐like ( AeIMUC1 ) and short‐chain dehydrogenase/reductase ( SDR ) genes as midgut‐expressed candidate genes influencing susceptibility to infection by Plasmodium gallinaceum (Brumpt). We used RNA inference (RNAi) by double‐stranded RNA (dsRNA) injections to examine ookinete survival to the oocyst stage following individual gene knock‐downs. Double‐stranded RNA gene knock‐downs were performed 3 days prior to P. gallinaceum infection and oocyst development was evaluated at 7 days post‐infection. Mean numbers of parasites developing to the oocyst stage were significantly reduced by 52.3% in dsAeIMUC1 ‐injected females and by 36.5% in dsSDR ‐injected females compared with females injected with a dsβ‐gal control. The prevalence of infection was significantly reduced in dsAeIMUC1 ‐ and dsSDR ‐injected females compared with females injected with dsβ‐gal ; these reductions resulted in a two‐ and three‐fold increase in the number of uninfected individuals, respectively. Overall, these results suggest that both AeIMUC1 and SDR play a role in Ae. aegypti vector competence to P. gallinaceum . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1365-2915.2011.00965.x |
| DOI: | 10.1111/j.1365-2915.2011.00965.x/fullpdf |
| الاتاحة: | http://dx.doi.org/10.1111/j.1365-2915.2011.00965.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2915.2011.00965.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2915.2011.00965.x/fullpdf |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.D5F05832 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1365-2915.2011.00965.x |
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