التفاصيل البيبلوغرافية
| العنوان: |
Structure and Dynamics of Human Chemokine CCL16—Implications for Biological Activity |
| المؤلفون: |
Oliver H. Weiergräber, Dušan Petrović, Andreas Kislat, Martin Pattky, Judith Fabig, Renu Batra-Safferling, Jan Schulte am Esch, Karen Hänel, Carolin Huhn, Birgit Strodel, Bernhard Homey, Dieter Willbold |
| المصدر: |
Biomolecules; Volume 12; Issue 11; Pages: 1588 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2022 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
CCL16, chemokine, glycosaminoglycan, haptotaxis, limited proteolysis |
| جغرافية الموضوع: |
agris |
| الوصف: |
Human C-C motif ligand 16 (CCL16) is a chemokine that is distinguished by a large cleavable C-terminal extension of unknown significance. Conflicting data have been reported concerning its tissue distribution and modulation of expression, rendering the biological function of CCL16 enigmatic. Here, we report an integrated approach to the characterisation of this chemokine, including a re-assessment of its expression characteristics as well as a biophysical investigation with respect to its structure and dynamics. Our data indicate that CCL16 is chiefly synthesised by hepatocytes, without an appreciable response to mediators of inflammation, and circulates in the blood as a full-length protein. While the crystal structure of CCL16 confirms the presence of a canonical chemokine domain, molecular dynamics simulations support the view that the C-terminal extension impairs the accessibility of the glycosaminoglycan binding sites and may thus serve as an intrinsic modulator of biological activity. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Molecular Structure and Dynamics; https://dx.doi.org/10.3390/biom12111588 |
| DOI: |
10.3390/biom12111588 |
| الاتاحة: |
https://doi.org/10.3390/biom12111588 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.D5B657C5 |
| قاعدة البيانات: |
BASE |