Unspliced, spliced, and total XBP1 semi-quantitative RT-PCR to identify endoplasmic reticulum stress.

التفاصيل البيبلوغرافية
العنوان: Unspliced, spliced, and total XBP1 semi-quantitative RT-PCR to identify endoplasmic reticulum stress.
المؤلفون: Edgar Joel Soto-Moreno (11820023), Ahmed Balboula (11820026), Christine Spinka (11820029), Rocío Melissa Rivera (4876195)
سنة النشر: 2021
المجموعة: Smithsonian Institution: Digital Repository
مصطلحات موضوعية: Medicine, Cell Biology, Genetics, Molecular Biology, Developmental Biology, Hematology, Plant Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, trophectoderm cell number, trophectoderm cell allocations, inner cell mass, gene expression alterations, er stress markers, endoplasmic reticulum stress, assessed transcript level, medium containing 10, examined blastocyst morphometry, bovine embryo culture, promote cell proliferation, div >< p, day 5 post, 16 cells embryos, serum increased diameter, preimplantation embryo development, day 8 blastocysts, serum increased macroautophagy, embryo diameter, day 8, day 5
الوصف: A. Unspliced, spliced, and total XBP1 ratios of single bovine embryos. B. Ratio of spliced XBP1 of tunicamycin treated, vehicle, and control embryos. TM treatment indeed shows splicing of XBP1, suggesting an UPR response to induced ER stress. TM = 5μg Tunicamycin. Vehicle = 0.05% ethanol alcohol. (TIF)
نوع الوثيقة: still image
اللغة: unknown
Relation: https://figshare.com/articles/figure/Unspliced_spliced_and_total_XBP1_semi-quantitative_RT-PCR_to_identify_endoplasmic_reticulum_stress_/17154320
DOI: 10.1371/journal.pone.0260123.s003
الاتاحة: https://doi.org/10.1371/journal.pone.0260123.s003
Rights: CC BY 4.0
رقم الانضمام: edsbas.D519AB91
قاعدة البيانات: BASE
الوصف
DOI:10.1371/journal.pone.0260123.s003