Academic Journal
Cytokine release after gluten ingestion differentiates coeliac disease from self‐reported gluten sensitivity
| العنوان: | Cytokine release after gluten ingestion differentiates coeliac disease from self‐reported gluten sensitivity |
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| المؤلفون: | Tye‐Din, Jason A, Skodje, Gry I, Sarna, Vikas K, Dzuris, John L, Russell, Amy K, Goel, Gautam, Wang, Suyue, Goldstein, Kaela E, Williams, Leslie J, Sollid, Ludvig M, Lundin, Knut EA, Anderson, Robert P |
| المساهمون: | EkstraStiftelsen Helse og Rehabilitering, Helse Sør-Øst RHF, Norges Forskningsråd, Stiftelsen Kristian Gerhard Jebsen, Universitetet i Oslo, South-Eastern Norway Health Authority, University of Oslo World-leading research program on human immunology, Centre of Excellence funding scheme of the Research Council of Norway, Stiftelsen KG Jebsen, Mathison Centenary Fellowship, University of Melbourne, ImmusanT Inc., Norwegian ExtraFoundation for Health and Rehabilitation |
| المصدر: | United European Gastroenterology Journal ; volume 8, issue 1, page 108-118 ; ISSN 2050-6406 2050-6414 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2020 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Background Diagnosing coeliac disease (CD) in patients on a gluten‐free diet (GFD) is difficult. Ingesting gluten elevates circulating interleukin (IL)‐2, IL‐8 and IL‐10 in CD patients on a GFD. Objective We tested whether cytokine release after gluten ingestion differentiates patients with CD from those with self‐reported gluten sensitivity (SR‐GS). Methods Australian patients with CD ( n = 26) and SR‐GS ( n = 18) on a GFD consumed bread (estimated gluten 6 g). Serum at baseline and at 3 and 4 h was tested for IL‐2, IL‐8 and IL‐10. Separately, Norwegian SR‐GS patients ( n = 49) had plasma cytokine assessment at baseline and at 2, 4 and 6 h after food bars containing gluten (5.7 g), fructan or placebo in a previous double‐blind crossover study. Results Gluten significantly elevated serum IL‐2, IL‐8 and IL‐10 at 3 and 4 h in patients with CD but not SR‐GS. The highest median fold‐change from baseline at 4 h was for IL‐2 (8.06, IQR: 1.52–24.0; P < 0.0001, Wilcoxon test). The two SR‐GS cohorts included only one (1.5%) confirmed IL‐2 responder, and cytokine responses to fructan and placebo were no different to gluten. Overall, cytokine release after gluten was present in 22 (85%) CD participants, but 2 of the 4 non‐responders remained clinically well after 1 y on an unrestricted diet. Hence, cytokine release occurred in 22 (92%) of 24 ‘verified’ CD participants. Conclusions Gluten challenge with high‐sensitivity cytokine assessment differentiates CD from SR‐GS in patients on a GFD and identifies patients likely to tolerate gluten reintroduction. Systemic cytokine release indicating early immune activation by gluten in CD individuals cannot be detected in SR‐GS individuals. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/2050640619874173 |
| الاتاحة: | http://dx.doi.org/10.1177/2050640619874173 https://onlinelibrary.wiley.com/doi/pdf/10.1177/2050640619874173 https://onlinelibrary.wiley.com/doi/full-xml/10.1177/2050640619874173 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor ; http://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.D4CCF0BF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/2050640619874173 |
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