Academic Journal
基于AuNPs/PANI/TNTs纳米复合材料的电化学检测妥布霉素的适配体传感器 ; An Aptasensor Based on AuNPs/PANI/TNTs Nanocomposite for Electrochemical Detection of Tobramycin
| العنوان: | 基于AuNPs/PANI/TNTs纳米复合材料的电化学检测妥布霉素的适配体传感器 ; An Aptasensor Based on AuNPs/PANI/TNTs Nanocomposite for Electrochemical Detection of Tobramycin |
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| المؤلفون: | 农永玲, 乔妮娜, 梁营, NONG Yong-ling, QIAO Ni-na, LIANG Ying |
| المصدر: | http://electrochem.xmu.edu.cn/CN/abstract/abstract10485.shtml. |
| بيانات النشر: | 厦门大学《电化学》编辑部 |
| سنة النشر: | 2019 |
| المجموعة: | Xiamen University Institutional Repository |
| مصطلحات موضوعية: | 适配体, 妥布霉素, 电化学分析, 金纳米粒子, aptasensor, tobramycin, electrochemical analysis, Au nanoparticles |
| الوصف: | 本文提出了一种新型的检测妥布霉素的电化学适配体传感器，以差分脉冲伏安法（DPV）作为检测技术，亚甲基蓝作为电化学响应信号. 构建了以纳米复合材料金纳米粒子/聚苯胺/二氧化钛纳米管(AuNPs/PANI/TNTs)修饰玻碳电极的电极支架. 通过透射电子显微镜和X-射线光电子能谱对纳米复合材料进行详细的表征. 循环伏安图和电化学阻抗谱显示AuNPs/PANI/TNTs可以很好地增加电极的界面传导性能. DPV结果显示电流密度的响应和妥布霉素浓度之间存在一个很好的线性关系，并且得到一个宽广的检测范围为0.5 μmol·L-1到70 μmol·L-1. 本文提出的适配体基的传感器有很好的重复性和稳定性，作为一个潜在的手段可以应用在生物分析和医疗诊断中. ; A novel well-constructed electrochemical aptamer-based sensor for the detection of tobramycin was presented, using differential pulse voltammetry (DPV) as a detection technique and methylene blue（MB） as an electrochemical indicator. A glassy carbon electrode modified with a nanocomposite of Au nanoparticles/polyaniline/titania nanotubes (AuNPs/PANI/TNTs) was constructed as the electrode scaffold. The nanocomposite was characterized by transmission electron microscopy and X-ray photoelectron spectroscopy in detail. The results of cyclic voltammetry and electrochemical impedance measurements demonstrated that the AuNPs/PANI/TNTs nanocomposites can improve greatly the electron transfer on the interface. For the detection of tobramycin, the DPV results showed a linear relationship between the current response and the concentration of tobramycin, and a wide range of detection from 0.5 μmol·L-1 to 70 μmol·L-1. The presented aptamer-based biosensor exhibited excellent sensitivity and reproducibility, which would have a potential application in bioanalysis and clinical diagnostics. ; The project was supported financially by the National Natural Science Foundation of China (No. 21201043). ; 作者联系地址：1. 医药化工学院, 广东药科大学, 广州 510006, 中国; 2. 广东省化妆品工程技术研究中心, 广州 510006, 中国 ; Author's Address: 1. School of Chemistry and Chemical Engineering, GuangDong Pharmaceutical University, Guangzhou, 510006, China; 2. Guangdong Cosmetics Engineering & Technology Research Center, Guangzhou, 510006, China ; 通讯作者E-mail：lyingair@126.com |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | Chinese |
| تدمد: | 1006-3471 |
| Relation: | 研究论文; Articles; 电化学,2019,25(06):720-730.; http://dx.doi.org/10.13208/j.electrochem.180612; https://dspace.xmu.edu.cn/handle/2288/174217 |
| DOI: | 10.13208/j.electrochem.180612 |
| الاتاحة: | https://doi.org/10.13208/j.electrochem.180612 https://dspace.xmu.edu.cn/handle/2288/174217 |
| رقم الانضمام: | edsbas.D4CC760 |
| قاعدة البيانات: | BASE |
| تدمد: | 10063471 |
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| DOI: | 10.13208/j.electrochem.180612 |