Academic Journal
Recent advances in the improvement of cyanobacterial enzymes for bioalkane production
| العنوان: | Recent advances in the improvement of cyanobacterial enzymes for bioalkane production |
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| المؤلفون: | Hayashi, Yuuki, Arai, Munehito |
| المساهمون: | Japan Society for the Promotion of Science, Japan, Yashima Environment Technology Foundation, Iketani Science and Technology Foundation |
| المصدر: | Microbial Cell Factories ; volume 21, issue 1 ; ISSN 1475-2859 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| الوصف: | The use of biologically produced alkanes has attracted considerable attention as an alternative energy source to petroleum. In 2010, the alkane synthesis pathway in cyanobacteria was found to include two small globular proteins, acyl-(acyl carrier protein [ACP]) reductase (AAR) and aldehyde deformylating oxygenase (ADO). AAR produces fatty aldehydes from acyl-ACPs/CoAs, which are then converted by ADO to alkanes/alkenes equivalent to diesel oil. This discovery has paved the way for alkane production by genetically modified organisms. Since then, many studies have investigated the reactions catalyzed by AAR and ADO. In this review, we first summarize recent findings on structures and catalytic mechanisms of AAR and ADO. We then outline the mechanism by which AAR and ADO form a complex and efficiently transfer the insoluble aldehyde produced by AAR to ADO. Furthermore, we describe recent advances in protein engineering studies on AAR and ADO to improve the efficiency of alkane production in genetically engineered microorganisms such as Escherichia coli and cyanobacteria. Finally, the role of alkanes in cyanobacteria and future perspectives for bioalkane production using AAR and ADO are discussed. This review provides strategies for improving the production of bioalkanes using AAR and ADO in cyanobacteria for enabling the production of carbon–neutral fuels. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s12934-022-01981-4 |
| DOI: | 10.1186/s12934-022-01981-4.pdf |
| DOI: | 10.1186/s12934-022-01981-4/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s12934-022-01981-4 https://link.springer.com/content/pdf/10.1186/s12934-022-01981-4.pdf https://link.springer.com/article/10.1186/s12934-022-01981-4/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.D49DCD64 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12934-022-01981-4 |
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