Academic Journal
Streptococcus pneumoniae Affects Endothelial Cell Migration in Microfluidic Circulation
| العنوان: | Streptococcus pneumoniae Affects Endothelial Cell Migration in Microfluidic Circulation |
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| المؤلفون: | Kopenhagen, Anna, Ramming, Isabell, Camp, Belinda, Hammerschmidt, Sven, Fulde, Marcus, Müsken, Mathias, Steinert, Michael, Bergmann, Simone |
| المصدر: | Frontiers in Microbiology 13 (2022) 852036. - https://doi.org/10.3389/fmicb.2022.852036 -- Front Microbiol -- https://www.ncbi.nlm.nih.gov/pmc/journals/1526/ -- http://www.frontiersin.org/microbiology -- http://www.bibliothek.uni-regensburg.de/ezeit/?2587354 -- 1664-302X -- 1664-302X |
| بيانات النشر: | Frontiers |
| سنة النشر: | 2022 |
| المجموعة: | Braunschweig Technical University: Braunschweig Digital Library |
| مصطلحات موضوعية: | Article, ddc:571, Veröffentlichung der TU Braunschweig, Publikationsfonds der TU Braunschweig, Endothelium, Wound healing, Streptococcus pneumoniae, cell migration, Microfluidic, Pneumolysin |
| الوصف: | Bloodstream infections caused by Streptococcus pneumoniae induce strong inflammatory and procoagulant cellular responses and affect the endothelial barrier of the vascular system. Bacterial virulence determinants, such as the cytotoxic pore-forming pneumolysin, increase the endothelial barrier permeability by inducing cell apoptosis and cell damage. As life-threatening consequences, disseminated intravascular coagulation followed by consumption coagulopathy and low blood pressure is described. With the aim to decipher the role of pneumolysin in endothelial damage and leakage of the vascular barrier in more detail, we established a chamber-separation cell migration assay (CSMA) used to illustrate endothelial wound healing upon bacterial infections. We used chambered inlets for cell cultivation, which, after removal, provide a cell-free area of 500 μm in diameter as a defined gap in primary endothelial cell layers. During the process of wound healing, the size of the cell-free area is decreasing due to cell migration and proliferation, which we quantitatively determined by microscopic live cell monitoring. In addition, differential immunofluorescence staining combined with confocal microscopy was used to morphologically characterize the effect of bacterial attachment on cell migration and the velocity of gap closure. In all assays, the presence of wild-type pneumococci significantly inhibited endothelial gap closure. Remarkably, even in the presence of pneumolysin-deficient pneumococci, cell migration was significantly retarded. Moreover, the inhibitory effect of pneumococci on the proportion of cell proliferation versus cell migration within the process of endothelial gap closure was assessed by implementation of a fluorescence-conjugated nucleoside analogon. We further combined the endothelial CSMA with a microfluidic pump system, which for the first time enabled the microscopic visualization and monitoring of endothelial gap closure in the presence of circulating bacteria at defined vascular shear stress values ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 15 Seiten |
| اللغة: | English |
| Relation: | https://doi.org/10.3389/fmicb.2022.852036; https://nbn-resolving.org/urn:nbn:de:gbv:084-2022062308367; https://leopard.tu-braunschweig.de/receive/dbbs_mods_00070844; https://leopard.tu-braunschweig.de/servlets/MCRFileNodeServlet/dbbs_derivate_00049499/Bergmann_fmicb-13-852036.pdf; http://publikationsserver.tu-braunschweig.de/get/70844; https://www.frontiersin.org/articles/10.3389/fmicb.2022.852036/pdf |
| DOI: | 10.3389/fmicb.2022.852036 |
| DOI: | 10.3389/fmicb.2022.852036/pdf |
| الاتاحة: | https://doi.org/10.3389/fmicb.2022.852036 https://nbn-resolving.org/urn:nbn:de:gbv:084-2022062308367 https://leopard.tu-braunschweig.de/receive/dbbs_mods_00070844 https://leopard.tu-braunschweig.de/servlets/MCRFileNodeServlet/dbbs_derivate_00049499/Bergmann_fmicb-13-852036.pdf http://publikationsserver.tu-braunschweig.de/get/70844 https://www.frontiersin.org/articles/10.3389/fmicb.2022.852036/pdf |
| Rights: | https://creativecommons.org/licenses/by/4.0/ ; public ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D49A4198 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fmicb.2022.852036 |
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