Dissertation/ Thesis
Monoclonal antibodies and blue marlin (Makaira nigricans) identification
| العنوان: | Monoclonal antibodies and blue marlin (Makaira nigricans) identification |
|---|---|
| المساهمون: | Paniker, Lakshmi., Florida Atlantic University (Degree grantor), Hartmann, James X. (Thesis advisor) |
| بيانات النشر: | Florida Atlantic University |
| المجموعة: | FAU Digital Collections (Florida Atlantic University Digital Library) |
| مصطلحات موضوعية: | Blue marlin--Identification, Fishes--Immunology, Immunology--Technique |
| الوصف: | Spleenocytes from Balb/c mouse immunized with purified blue marlin albumin were fused with X 63 myeloma cells in a polyethylene glycol aided fusion. The resulting fusomas were screened for anti-blue marlin activity and a potential blue marlin specific hybridoma clone designated 605D was isolated and expanded in cell culture. While testing the reactivity of monoclonal antibody secreted by clone 605D against pooled serum samples of blue marlin, white marlin and sailfish in indirect ELISA, 605D could differentiate blue marlin from white marlin and sailfish clearly. The crossreactivity to white marlin was minimised by diluting sera samples and antibody solutions. However, when individual billfish serum samples and heart tissue homogenates were tested 605D could not differentiate between the two marlin species, though crossreactivity to SF was minimal. ; Charles E. Schmidt College of Science ; Thesis (M.S.)--Florida Atlantic University, 1996. |
| نوع الوثيقة: | thesis |
| وصف الملف: | 57 p.; application/pdf |
| اللغة: | English |
| الاتاحة: | http://purl.flvc.org/fcla/dt/15347 https://fau.digital.flvc.org/islandora/object/fau%3A12115/datastream/TN/view/Monoclonal%20antibodies%20and%20blue%20marlin%20%28Makaira%20nigricans%29%20identification.jpg |
| Rights: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. ; http://rightsstatements.org/vocab/InC/1.0/ |
| رقم الانضمام: | edsbas.D4730475 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |