التفاصيل البيبلوغرافية
| العنوان: |
Examples of Using of Electrochemical Detection at Pencil Graphite Electrode with Enzymatic Labeling for Analysis of Nucleotide Sequence |
| المؤلفون: |
Horakova, Petra, Pivonkova, Hana, Ecsin, Ece, Erdem, Arzum, Fojta, Miroslav, Haronikova, Lucia, Plucnara, Medard, Spacek, Jan, Havran, Ludek |
| بيانات النشر: |
Lenka Srsenova-Best Servis |
| سنة النشر: |
2015 |
| المجموعة: |
Ege University Institutional Repository |
| مصطلحات موضوعية: |
Alkaline phosphatase, Enzymatic labeling, Single nucleotide polymorphism, Pencil graphite electrode |
| الوصف: |
WOS: 000358792900040 ; International Conference on Modern Electrochemical Methods XXXV -- MAY 18-22, 2015 -- Jetrichovice, CZECH REPUBLIC ; Many examples of utilization of enzymatic labeling for DNA sequence analysis has been described in literature so far. Some of them involve hybridization with complementary biotinylated probe, while others use incorporation of biotinylated nucleotides into DNA strand by DNA polymerases. Common approach is then binding of streptavidine-enzyme conjugates to biotin tags and incubation with substrate, which is converted to detectable product. Here, two recent applications using this principle are described for the detection of PCR amplicons and for SNP typing. Both techniques are combined with detection at pencil graphite electrodes. ; Charles Univ, Fac Sci, Dept Analyt Chem, UNESCO Lab Environm Electrochemistry, Inst Biophys |
| نوع الوثيقة: |
conference object |
| اللغة: |
Czech |
| Relation: |
Konferans Öğesi - Uluslararası - Kurum Öğretim Elemanı; Xxxv Moderni Elektrochemicke Metody; https://hdl.handle.net/11454/51125; 175; 179 |
| الاتاحة: |
https://hdl.handle.net/11454/51125 |
| Rights: |
none |
| رقم الانضمام: |
edsbas.D46BA5B6 |
| قاعدة البيانات: |
BASE |