Academic Journal
Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production
| العنوان: | Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production |
|---|---|
| المؤلفون: | Saaret, Annica, Villiers, Benoît, Stricher, François, Anissimova, Macha, Cadillon, Mélodie, Spiess, Reynard, Hay, Sam, Leys, David |
| المصدر: | eissn: 2041-1723 |
| بيانات النشر: | Nature Publishing Group UK |
| سنة النشر: | 2021 |
| المجموعة: | University of Chester: Chester Digital Repository |
| مصطلحات موضوعية: | Article, /631/45/603, /631/61/318, /639/638/224/906, /119/118, /145, /82/80 |
| الوصف: | From Springer Nature via Jisc Publications Router ; History: received 2021-03-02, accepted 2021-07-29, registration 2021-08-20, pub-electronic 2021-09-06, online 2021-09-06, collection 2021-12 ; Publication status: Published ; Funder: EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council); doi: https://doi.org/10.13039/100010663; Grant(s): pre-FAB 695013 ; Abstract: Isobutene is a high value gaseous alkene used as fuel additive and a chemical building block. As an alternative to fossil fuel derived isobutene, we here develop a modified mevalonate pathway for the production of isobutene from glucose in vivo. The final step in the pathway consists of the decarboxylation of 3-methylcrotonic acid, catalysed by an evolved ferulic acid decarboxylase (Fdc) enzyme. Fdc belongs to the prFMN-dependent UbiD enzyme family that catalyses reversible decarboxylation of (hetero)aromatic acids or acrylic acids with extended conjugation. Following a screen of an Fdc library for inherent 3-methylcrotonic acid decarboxylase activity, directed evolution yields variants with up to an 80-fold increase in activity. Crystal structures of the evolved variants reveal that changes in the substrate binding pocket are responsible for increased selectivity. Solution and computational studies suggest that isobutene cycloelimination is rate limiting and strictly dependent on presence of the 3-methyl group. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://chesterrep.openrepository.com/bitstream/handle/10034/625796/41467_2021_25598_MOESM2_ESM.pdf?sequence=2; https://chesterrep.openrepository.com/bitstream/handle/10034/625796/additional-files.zip?sequence=3; https://chesterrep.openrepository.com/bitstream/handle/10034/625796/41467_2021_Article_25598.pdf?sequence=4; https://chesterrep.openrepository.com/bitstream/handle/10034/625796/41467_2021_Article_25598_nlm.xml?sequence=5; https://chesterrep.openrepository.com/bitstream/handle/10034/625796/41467_2021_25598_MOESM3_ESM.pdf?sequence=6; https://chesterrep.openrepository.com/bitstream/handle/10034/625796/41467_2021_25598_MOESM1_ESM.pdf?sequence=7; Nature Communications, volume 12, issue 1, page 5300; http://hdl.handle.net/10034/625796 |
| الاتاحة: | http://hdl.handle.net/10034/625796 https://chesterrep.openrepository.com/bitstream/handle/10034/625796/41467_2021_25598_MOESM2_ESM.pdf?sequence=2 https://chesterrep.openrepository.com/bitstream/handle/10034/625796/additional-files.zip?sequence=3 https://chesterrep.openrepository.com/bitstream/handle/10034/625796/41467_2021_Article_25598.pdf?sequence=4 https://chesterrep.openrepository.com/bitstream/handle/10034/625796/41467_2021_Article_25598_nlm.xml?sequence=5 https://chesterrep.openrepository.com/bitstream/handle/10034/625796/41467_2021_25598_MOESM3_ESM.pdf?sequence=6 https://chesterrep.openrepository.com/bitstream/handle/10034/625796/41467_2021_25598_MOESM1_ESM.pdf?sequence=7 |
| Rights: | Licence for this article: http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.D4130B6B |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |