Academic Journal
Competition between neighbouring topogenic signals during membrane protein insertion into the ER membrane
| العنوان: | Competition between neighbouring topogenic signals during membrane protein insertion into the ER membrane |
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| المؤلفون: | MONNE', MAGNUS LUDVIG, T. HESSA, L. THISSEN, G. VON HEIJNE |
| المساهمون: | Monne', MAGNUS LUDVIG, T., Hessa, L., Thissen, G., VON HEIJNE |
| سنة النشر: | 2005 |
| المجموعة: | Università degli Studi della Basilicata: CINECA IRIS |
| مصطلحات موضوعية: | endoplasmic reticulum, kinetic, membrane protein, positive inside rule, topology |
| الوصف: | To better define the mechanism of membrane protein insertion into the membrane of the endoplasmic reticulum, we measured the kinetics of translocation across microsomal membranes of the N-terminal lumenal tail and the lumenal domain following the second transmembrane segment (TM2) in the multispanning mouse protein Cig30. In the wild-type protein, the N-terminal tail translocates across the membrane before the downstream lumenal domain. Addition of positively charged residues to the N-terminal tail dramatically slows down its translocation and allows the downstream lumenal domain to translocate at the same time as or even before the N-tail. When TM2 is deleted, or when the loop between TM1 and TM2 is lengthened, addition of positively charged residues to the N-terminal tail causes TM1 to adopt an orientation with its N-terminal end in the cytoplasm. We suggest that the topology of the TM1-TM2 region of Cig30 depends on a competition between TM1 and TM2 such that the transmembrane segment that inserts first into the ER membrane determines the final topology. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000227358000003; volume:272; issue:1; firstpage:28; lastpage:36; numberofpages:9; journal:THE FEBS JOURNAL; http://hdl.handle.net/11563/8925; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-11444249293 |
| DOI: | 10.1111/j.1432-1033.2004.04394.x |
| الاتاحة: | http://hdl.handle.net/11563/8925 https://doi.org/10.1111/j.1432-1033.2004.04394.x |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D3C58953 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1432-1033.2004.04394.x |
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