Academic Journal
Comparing two quantitative methods for studying remineralization of artificial caries
| العنوان: | Comparing two quantitative methods for studying remineralization of artificial caries |
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| المؤلفون: | Lo, ECM, Itthagarun, A, Zhi, QH |
| بيانات النشر: | //www.elsevier.com/locate/jdent United Kingdom |
| سنة النشر: | 2010 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | Remineralization, Dental caries, Polarized light microscopy, MicroCT, Transverse microradiography, Dental Enamel - pathology, Dental Caries - pathology - therapy, Dentin - pathology, Diagnostic Imaging - methods, Tooth Remineralization - methods |
| الوصف: | Objectives: To compare the detection of changes before and after remineralization of artificial enamel and dentin caries by microCT scanning, polarized light microscopy (PLM) and transverse microradiography (TMR). Methods: Fourteen extracted premolars were cut into tooth blocks and painted with an acid-resistant varnish leaving one enamel and one dentin surface exposed. The tooth blocks were immersed into demineralizing solution for 4 days to produce artificial caries-like lesions and scanned by microCT. Then the 14 tooth blocks were randomly allocated into two groups. Seven tooth blocks in Group I were cut longitudinally through the exposed surface into 100-150 μm thick sections and microradiographs were taken. The other seven tooth blocks in Group II were left intact. All the tooth blocks and sections were then immersed into remineralizing solution for 5 days. PLM and TMR of the tooth sections in Group I were taken again. Depth of the lesion on the TMR was measured. Tooth blocks in Group II were scanned by microCT. Results: Mean lesion depth in Group I reduced by 13.0% and 8.2% after remineralization for enamel and dentin, respectively (paired t-test, P < 0.001). In Group II, linear attenuation coefficient (LAC) of the region of interest (ROI) increased by 11.1% and 23.8% after remineralization for enamel and dentin lesions, respectively (paired t-test, P < 0.001). Conclusion: Both microCT and microradiography are able to detect a change of similar magnitude in the artificial caries lesions after remineralization. MicroCT may be used to substitute TMR and PLM in in vitro studies about caries. © 2010 Elsevier Ltd. All rights reserved. ; link_to_subscribed_fulltext |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1879-176X 0300-5712 |
| Relation: | Journal of Dentistry; http://www.scopus.com/mlt/select.url?eid=2-s2.0-77649191102&selection=ref&src=s&origin=recordpage; Journal Of Dentistry, 2010, v. 38 n. 4, p. 352-359; 6554404; 359; 174422; WOS:000276118500012; http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-5712&volume=38&issue=4&spage=352&epage=359&date=2010&atitle=Comparing+two+quantitative+methods+for+studying+remineralization+of+artificial+caries; eid_2-s2.0-77649191102; 352; http://hdl.handle.net/10722/124440; 38 |
| DOI: | 10.1016/j.jdent.2010.01.001 |
| الاتاحة: | https://doi.org/10.1016/j.jdent.2010.01.001 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-5712&volume=38&issue=4&spage=352&epage=359&date=2010&atitle=Comparing+two+quantitative+methods+for+studying+remineralization+of+artificial+caries http://hdl.handle.net/10722/124440 |
| رقم الانضمام: | edsbas.D379B721 |
| قاعدة البيانات: | BASE |
| تدمد: | 1879176X 03005712 |
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| DOI: | 10.1016/j.jdent.2010.01.001 |